@misc{10481/91044,
year = {2023},
month = {12},
url = {https://hdl.handle.net/10481/91044},
abstract = {Nanotechnological platforms offer advantages over conventional
therapeutic and diagnostic modalities. However, the efficient biointerfacing of
nanomaterials for biomedical applications remains challenging. In recent years,
nanoparticles (NPs) with different coatings have been developed to reduce
nonspecific interactions, prolong circulation time, and improve therapeutic
outcomes. This study aims to compare various NP coatings to enhance surface
engineering for more effective nanomedicines. We prepared and characterized
polystyrene NPs with different coatings of poly(ethylene glycol), bovine serum
albumin, chitosan, and cell membranes from a human breast cancer cell line. The
coating was found to affect the colloidal stability, adhesion, and elastic modulus of
NPs. Protein corona formation and cellular uptake of NPs were also investigated,
and a 3D tumor model was employed to provide a more realistic representation
of the tumor microenvironment. The prepared NPs were found to reduce protein
adsorption, and cell-membrane-coated NPs showed significantly higher cellular uptake. The secretion of proinflammatory cytokines
in human monocytes after incubation with the prepared NPs was evaluated. Overall, the study demonstrates the importance of
coatings in affecting the behavior and interaction of nanosystems with biological entities. The findings provide insight into bionano
interactions and are important for the effective implementation of stealth surface engineering designs.},
organization = {MCIN/AEI/10.13039/501100011033/
FEDER “Una manera de hacer Europa” for funding
RTI2018.101309B-C21, RTI2018.101309B−C22, PID2022-
140151OB-C21, PID2022-140151OB-C22, and PID2021-
124363OA-I00 projects},
organization = {Chair “Doctors Galera-
Requena in cancer stem cell research”},
organization = {University of Granada Research plan for funding PPJIA2021.21 project},
organization = {Ph.D. student fellowship
FPU18/05336 PRE2019-088029 and FPU19/02045},
organization = {Funding for open access charge: Universidad de
Granada/CBUA},
publisher = {American Chemical Society},
keywords = {Nanoparticles},
keywords = {Coatings},
keywords = {Cell membranes},
title = {Exploring the Impact of Nanoparticle Stealth Coatings in Cancer Models: From PEGylation to Cell Membrane-Coating Nanotechnology},
doi = {10.1021/acsami.3c13948},
author = {Graván, Pablo and Peña Martín, Jesús and López de Andrés, Julia and Pedrosa Bustos, María and Villegas Montoya, Martín and Galisteo González, Francisco and Marchal Corrales, Juan Antonio and Sánchez Moreno, Paola},
}