@misc{10481/90578,
year = {2024},
url = {https://hdl.handle.net/10481/90578},
abstract = {The health benefits of chestnut (Castanea sativa) shells (CSs) have been ascribed to phytochemicals, mainly
phenolic compounds. Nevertheless, an exhaustive assessment of their intestinal absorption is vital considering a
possible nutraceutical application. This study evaluated the bioactivity of CSs extract prepared by Supercritical
Fluid Extraction and untargeted metabolomic profile upon in-vitro intestinal permeation across a Caco-2/HT29-
MTX co-culture model. The results demonstrated the neuroprotective, hypoglycemic, and hypolipidemic properties
of CSs extract by inhibition of acetylcholinesterase, α-amylase, and lipase activities. The untargeted
metabolic profiling by LC-ESI-LTQ-Orbitrap-MS unveiled almost 60 % of lipids and 30 % of phenolic compounds,
with 29 metabolic pathways indicated by enrichment analysis. Among phenolics, mostly phenolic acids, flavonoids,
and coumarins permeated the intestinal barrier with most metabolites arising from phase I reactions
(reduction, hydrolysis, and hydrogenation) and a minor fraction from phase II reactions (methylation). The
permeation rates enhanced in the following order: ellagic acid < o-coumaric acid < p-coumaric acid < ferulaldehyde
≤ hydroxyferulic acid ≤ dihydroferulic acid < ferulic acid < trans-caffeic acid < trans-cinnamic acid
< dihydrocaffeic acid, with better outcomes for 1000 μg/mL of extract concentration and after 4 h of permeation.
Taken together, these findings sustained a considerable in-vitro intestinal absorption of phenolic compounds from
CSs extract, enabling them to reach target sites and exert their biological effects.},
organization = {National funds
(UIDB/50006/2020)},
organization = {Project PTDC/ASP-AGR/29277/2017 - Castanea sativa shells as a new source of active ingredients for Functional Food and Cosmetic applications: a sustainable approach, and project 5537 DRI, Sérvia 2020/21 from Portuguese-Serbia Bilateral Cooperation - Development of functional foods incorporating a chestnut shells extract obtained by subcritical water, supported by national funds by FCT/ MCTES and co-supported by Fundo Europeu de Desenvolvimento Regional (FEDER) throughout COMPETE 2020 - Programa Operacional Competitividade e Internacionalização (POCI-01-0145-FEDER-029277)},
organization = {PhD grants financed by FCT/
MCTES and POPH-QREN (SFRH/BD/144534/2019 and SFRH/
BD/144994/2019)},
organization = {Funds from European Union
(EU) and Fundo Social Europeu (FSE) through Programa Operacional
Regional Norte},
organization = {Contract (CEECIND/
01886/2020) financed by FCT/MCTES—CEEC Individual 2020
Program Contract},
publisher = {Elsevier},
keywords = {Castanea sativa},
keywords = {Phenolic compounds},
keywords = {Supercritical fluid extraction},
title = {Novel insights into enzymes inhibitory responses and metabolomic profile of supercritical fluid extract from chestnut shells upon intestinal permeability},
doi = {10.1016/j.foodres.2023.113807},
author = {Pinto, Diana and Lozano Castellón, Julián and Silva, Ana Margarida and Cádiz Gurrea, María de la Luz and Segura Carretero, Antonio and Lamuela-Raventós, Rosa María and Vallverdú Queralt, Anna and Delerue Matos, Cristina and Rodrigues, Francisca},
}