@misc{10481/90536,
year = {2024},
url = {https://hdl.handle.net/10481/90536},
abstract = {The disinfection of drinking water generates hundreds of disinfection byproducts (DBPs), including haloaromatic
DBPs. These haloaromatic DBPs are suspected to be more toxic than haloaliphatic ones, and they are currently
not regulated. This work investigates their toxicity and ability to interfere with estrogen synthesis in human
placental JEG-3 cells, and their genotoxic potential in human alveolar A549 cells. Among the haloaromatic DBPs
studied, halobenzoquinones (2,6-dichloro-1,4-benzoquinone (DCBQ) and 2,6-dibromo-1,4-benzoquinone
(DBBQ)) showed the highest cytotoxicity (EC50: 18–26 μg/mL). They induced the generation of very high
levels of reactive oxygen species (ROS) and up-regulated the expression of genes involved in estrogen synthesis
(cyp19a1, hsd17b1). Increased ROS was linked to significant depletion of polyunsaturated lipid species from inner
cell membranes. The other DBPs tested showed low or no significant cytotoxicity (EC50 ≥ 100 μg/mL), while
2,4,6-trichloro-phenol (TCP), 2,4,6-tribromo-phenol (TBP) and 3,5-dibromo-4-hydroxybenzaldehyde (DCHB)
induced the formation of micronuclei at concentrations much higher than those typically found in water (100 μg/
mL). This study reveals the different modes of action of haloaromatic DBPs, and highlights the toxic potential of
halobenzoquinones, which had a significant impact on the expression of placenta steroid metabolism related
genes and induce oxidative stress, implying potential adverse health effects.},
organization = {Fundación General del CSIC
through the ComFuturo Programme (2nd edition)},
organization = {Grant RYC2020
-028901-I funded by MCIN/AEI/10.13039/501100011033 and “ESF
investing in your future”},
organization = {“Agencia Estatal
de Investigación” from the Spanish Ministry of Science and Innovation},
organization = {IDAEA-CSIC, a Centre of Excellence Severo Ochoa (CEX2018-
000794-S)},
organization = {Project PID2021-122592NB-I00 (Ministerio de Ciencia e
Innovación)},
publisher = {Elsevier},
keywords = {Halobenzoquinones},
keywords = {Water disinfection},
keywords = {Reprotoxicity},
title = {Non-regulated haloaromatic water disinfection byproducts act as endocrine and lipid disrupters in human placental cells},
doi = {10.1016/j.envpol.2023.123092},
author = {Pérez-Albaladejo, Elisabet and Casado, Marta and Postigo, Cristina and Porte, Cinta},
}