@misc{10481/90340,
year = {2023},
url = {https://hdl.handle.net/10481/90340},
abstract = {Besides canonical microRNAs (miRNAs), sequence-based variations called iso-miRs
have biological relevance and diagnostic potential; however, accurate
calling of these post-transcriptional modifications is challenging, especially for
low input samples. Here, we present IsoSeek, a sequencing protocol that
reduces ligation and PCR amplification bias and improves the accuracy of miRNA
detection in low input samples. We describe steps for using randomized adapters
combined with unique molecular identifiers (UMI), library quantification, and
sequencing, followed by detailed procedures for data processing and analysis.},
organization = {Multiple grants awarded to D.M.P. including NWO Perspectief Cancer-ID, TKI-Health Holland AQrate, and Cancer Center Amsterdam Foundation},
publisher = {Elsevier},
title = {IsoSeek for unbiased and UMI-informed sequencing of miRNAs from low input samples at single-nucleotide resolution},
doi = {10.1016/j.xpro.2023.102645},
author = {van Eijndhoven, Monique A.J. and Scheepbouwer, Chantal and Aparicio Puerta, Ernesto and Hackenberg, Michael and Pegtel, D. Michiel},
}