@misc{10481/89915,
year = {2022},
url = {https://hdl.handle.net/10481/89915},
abstract = {Hydrogels are polymeric biomaterials characterised by their promising biological and biomechanical
properties, which make them potential alternatives for use in tendon repair. The aim of the present study
was to generate in vitro, and determine the therapeutic efficacy in vivo, of novel nanostructured fibrin-based
hydrogels to be used as an augmentation strategy for the surgical repair of rat Achilles tendon injuries. Fibrin,
fibrin-agarose and fibrin-collagen nanostructured hydrogels (NFH, NFAH and NFCH, respectively) were
generated and their biomechanical properties and cell-biomaterial interactions characterised ex vivo. Achilles
tendon ruptures were created in 24 adult Wistar rats, which were next treated with direct repair (control
group) or direct repair augmented with the generated biomaterials (6 rats/group). After 4 and 8 weeks, the
animals were euthanised for macroscopical and histological analyses. Biomechanical characterisation showed
optimal properties of the biomaterials for use in tendon repair. Moreover, biological analyses confirmed
that tendon-derived fibroblasts were able to adhere to the surface of the generated biomaterials, with high
levels of viability and functionality. In vivo studies demonstrated successful tendon repair in all groups.
Lastly, histological analyses disclosed better tissue and extracellular matrix organisation and alignment with
biomaterial-based augmentation strategies than direct repair, especially when NFAH and NFCH were used.
The present study demonstrated that nanostructured fibrin-collagen hydrogels can be used to enhance the
healing process in the surgical repair of tendon ruptures.},
organization = {The study was supported by the Spanish Society of Orthopaedics and Traumatology (SECOT), the Spanish Plan Nacional de Investigación Científica, Desarrollo e Innovación Tecnológica, Ministerio de Economía y Competitividad (Instituto de Salud Carlos III), the European Regional Development Fund (ERDF-FEDER) Grant number FIS PI20-0318 and the Grant number P18-RT-5059 from the Plan Andaluz de Investigación, Desarrollo e Innovación (PAIDI 2020), Consejería de Transformación Económica, Industria, Conocimiento y Universidades, Andalusian Regional Government, Spain. The authors are grateful to Dr Ariane Ruyffelaert for her advice on the English version of the manuscript and to Karen Shashok for editing the revised manuscript. The authors are also grateful to Amalia de la Rosa Romero and Concepción López Rodríguez (Experimental Unit, University Hospital Virgen de las Nieves, Granada, Spain) and Fabiola Bermejo Casares (Department of Histology, University of Granada, Spain) for their technical assistance.},
publisher = {AO Research Institute Davos},
keywords = {Tissue engineering},
keywords = {Tendon},
keywords = {Fibrin-agarose hydrogels},
keywords = {Natural biomaterials},
keywords = {Regeneration (Biology)},
keywords = {Orthopaedics},
keywords = {Fibrin},
keywords = {Hydrogels},
keywords = {Ingeniería tisular},
title = {Nanostructured fibrin-based hydrogel membranes for use as an augmentation strategy in achilles tendon surgical repair in rats},
doi = {10.22203/eCM.v043a13},
author = {González Quevedo, David and Sánchez Porras, David and García García, Óscar Darío and Chato Astrain, Jesús and Diaz-Ramos, M and Campos Muñoz, Antonio Jesús and Carriel Araya, Víctor and Campos Sánchez, Fernando},
}