@misc{10481/89813,
year = {2018},
month = {2},
url = {https://hdl.handle.net/10481/89813},
abstract = {Generation of biomimetic and biocompatible artificial tissues is the basic research objective for tissue engineering (TE). In this sense, the biofabrication of scaffolds that resemble the tissues’ extracellular matrix (ECM) is an essential aim in this field. Uncompressed and nanostructured fibrin-agarose hydrogels (FAH and NFAH respectively) emerged as promising scaffold in TE, but its structure and biomechanical properties must be improved in order to broad their TE applications. Here we generated and characterized novel membranelike models with increased structural and biomechanical properties based on the chemical cross-linking of FAH and NFAH with genipin (GP at 0.1, 0.25, 0.5 and 0.75%). Furthermore, scaffolds were subjected to rheological (G, G’, G” modulus), ultrastructural and ex vivo biocompatibility analyses. Results showed that all GP concentrations increased the stiffness (G) and especially the elasticity (G’) of FAH and NFAH. Ultrastructural analyses demonstrated that GP and nanostructuration of FAH allowed controlling the porosity of FAH. In addition, biological studies revealed that higher concentration of GP significantly decreased the cell viability. Finally, this study demonstrated the possibility to generate natural FAH and NFAH with improved structural and biomechanical properties by using GP. However, further in vivo studies are needed in order to demonstrate the biocompatibility, biodegradability and regeneration capability of these cross-linked scaffolds.},
keywords = {Tissue Engineering},
keywords = {Nanostructuration technique},
keywords = {Genipin Cross-linking},
keywords = {Fibrin-agarose},
keywords = {Hydrogels},
keywords = {Cell-biomaterials interactions},
keywords = {Rheology},
title = {Generation of genipin cross-linked fibrin-agarose hydrogel tissue-like models for tissue engineering applications},
doi = {10.1088/1748-605X/aa9ad2},
author = {Campos Sánchez, Fernando and Bonhome Espinosa, Ana Belén and Vizcaíno, G and Rodríguez, IA and Durán-Herrera, D and López López, Modesto Torcuato and Sánchez-Montesinos García, Indalecio and Alaminos Mingorance, Miguel and Sánchez Quevedo, María del Carmen and Carriel Araya, Víctor},
}