@misc{10481/88921,
year = {2023},
url = {https://hdl.handle.net/10481/88921},
abstract = {Taiwaniaquinoids are a unique family of diterpenoids predominantly isolated from Taiwania cryptomerioides Hayata. Previously, we evaluated the antiproliferative effect of several synthetic taiwaniaquinoids against human lung (A-549), colon (T-84), and breast (MCF-7) tumor cell lines. Herein, we report the in vitro and in vivo antitumor activity of the most potent compounds. Their cytotoxic activity against healthy peripheral blood mononuclear cells (PBMCs) has also been examined. We underscore the limited toxicity of compound C36 in PBMCs and demonstrate that it exerts its antitumor effect in MCF-7 cells (IC50 = 1.8 µM) by triggering an increase in reactive oxygen species, increasing the cell population in the sub-G1 phase of the cell cycle (90 %), and ultimately activating apoptotic (49.6 %) rather than autophagic processes. Western blot results suggested that the underlying mechanism of the C36 apoptotic effects was linked to caspase 9 activation and a rise in the Bax/Bcl-2 ratio. In vivo analyses showed normal behavior and hematological parameters in C57BL/6 mice post C36 treatment. Moreover, no significant impact was observed on the biochemical parameters of these animals, indicating that C36 did not induce liver toxicity. Furthermore, C36 demonstrated a significant reduction in tumor growth in immune-competent C57BL/6 mice implanted with E0771 mouse mammary tumor cells, effectively improving survival rates. These findings position taiwaniaquinoids, particularly compound C36, as promising therapeutic candidates for human breast cancer.},
organization = {Consejería de Salud y Familias - Junta de Andalucía (PECART-0207-2020), the Junta de Andalucía - Universidad de Granada - European Regional Development Fund (B‐FQM‐278–UGR20), and the Ministerio de Economía y Competitividad (CTQ2014-56611-R).},
keywords = {Taiwaniaquinoids},
keywords = {Cancer},
keywords = {Apoptosis},
keywords = {Cell cycle arrest},
keywords = {Oxidative stress},
keywords = {In vivo},
title = {Discovery of a synthetic taiwaniaquinoid with potent in vitro and in vivo antitumor activity against breast cancer cells},
doi = {10.1016/j.biopha.2023.115791},
author = {Mut-Salud, Nuria and Guardia, Juan José and Fernández, Antonio and Blancas López-Barajas, María Isabel and Zentar, Houda and Garrido Jiménez, José Manuel and Álvarez De Manzaneda Roldán, Enrique and Chahboun Karimi, Rachid and Rodríguez Serrano, Fernando},
}