@misc{10481/88440,
year = {2002},
url = {https://hdl.handle.net/10481/88440},
abstract = {As observed for most eukaryotic cells, trypanosomatids contains non-LTR retrotransposons randomly inserted in the nuclear
genome. Autonomous retroelements which, code for their own transposition, have been characterized in Trypanosoma brucei (ingi)
and Trypanosoma cruzi (L1Tc), whereas non-autonomous retroelements have only been characterized in T. brucei (RIME). Here,
we have characterized in the genome of Trypanosoma cruzi four complete copies of a non-autonomous non-LTR retrotransposon,
called NARTc. This 0.26 kb NARTc element has the characteristics of non-LTR retrotransposons: the presence a poly(dA) tail and
of a short flanking duplicated motif. Analysis of the Genome Survey Sequence databases indicated that the Trypanosoma cruzi
haploid genome contains about 140 NARTc copies and about twice as many L1Tc copies. Interestingly, the NARTc and L1Tc
retroelements share, with the Trypanosoma brucei ingi and RIME retrotransposons, a common sequence (the first 45 bp with 91%
identity), whereas the remaining sequences are very divergent. This suggests that these four trypanosome non-LTR retrotransposons
were derived from the same common ancester and the sequence of their 5’-extremity may have a functional role. In addition, the
genome of Leishmania major contains the same conserved motif present in the trypanosome retroelements, whicle no transposable
elements have been detected so far in Leishmania sp},
organization = {We are extremely grateful to the Instituto de Investigaciones
Biotecnolo´ gicas, Buenos Aires, Argentina
(IIB INTECH*/http://cruzi.unsam.edu.ar/gss.html*/
cfrasch@iib.unsam.edu.ar) and the Instituto de Parasitologia
y Biomedicina, Granada, Spain (IPBLN), for
all the GSS and EST sequences released on the T. cruzi
database (http://www.ebi.ac.uk/blast2/parasites.html),
and the Wellcome Trust Sanger Institute (http://
www.sanger.ac.uk/Projects/L_major) for the DNA
genomic sequences released on the L. major database.
We also would like to thank M. Levin for useful
discussion all along this analysis. This work was
supported by the CNRS, the Conseil Re´gional d’Aquitaine,
the GDR Parasitologie (CNRS), the Ministe`re de
l’Education Nationale de la Recherche et de la Technologie
(Action Microbiologie). S.R. Heras, J.L. Garcı´a-
Pe´rez, M.C. Lo´pez were supported by BFM2000-1381
from MCyT, Spain. N.M. El-Sayed and E. Ghedin were
supported by NIH grant U01-AI45038 (NES).},
publisher = {Elsevier},
title = {Identification of non-autonomous non-LTR retrotransposons in the genome of Trypanosoma cruzi},
doi = {10.1016/s0166-6851(02)00167-6},
author = {Bringaud, Frédéric and Garcı́a-Pérez, José Luis and Rodríguez Heras, Sara and López, Manuel C},
}