@misc{10481/87640,
year = {2012},
month = {6},
url = {https://hdl.handle.net/10481/87640},
abstract = {Polyphenols from extra virgin olive oil (EVOO), a main component of the Mediterranean diet, have demonstrated
repeatedly anti-tumor activity in several in vitro and in vivo studies. However, little is known
about the efficiency of the absorption process and metabolic conversion of these compounds at cellular
level. In this study, a nano liquid chromatography–electrospray ionization–time of flight mass spectrometry
(nanoLC–ESI–TOF MS) method was developed to study the cellular uptake and metabolism of
olive oil phenols in JIMT-1 human breast cancer cells. After incubation for different time periods with
EVOO-derived phenolic extracts, culture media, cytosolic fraction and solid particles fraction were separated
and analyzed. Most of the free phenols, mainly hydroxytyrosol, its secoiridoid derivatives, and
the flavonoid luteolin, disappeared in the culture media in different ways and at different times. Besides,
several metabolites were detected in the culture media, fact that may indicate absorption and intracellular
metabolism followed by rapid cellular export. Low intracellular accumulation was observed with
only traces of some compounds detected in the cytosolic and solid particles fractions. Methylated conjugates
were the major metabolites detected, suggesting a catalytic action of catechol-O-methyl transferase
(COMT) in cancer cells.},
organization = {The authors are very grateful to Ministry of Education and Science (FPU, AP2005-4356) and Junta de Andalucia (project P09-FQM-5469, project P07-AGR-02619 and AGL 2008-05108- CO3-03/ALI). Besides, this work was supported in part by Instituto de Salud Carlos III (Ministerio de Sanidad y Consumo, Fondo de Investigación Sanitaria – FIS, Spain, Grants CP05-00090, PI06-0778 and RD06-0020-0028).},
publisher = {Elsevier},
title = {Uptake and metabolism of olive oil polyphenols in human breast cancer cells using nano-liquid chromatography coupled to electrospray ionization–time of flight-mass spectrometry},
doi = {10.1016/j.jchromb.2012.04.021},
author = {García Villalba, Rocío and Carrasco-Pancorbo, Alegria and Oliveras-Ferraros, Cristina and Menéndez, Javier A and Segura-Carretero, Antonio and Fernández-Gutiérrez, Alberto},
}