@misc{10481/86531,
year = {2021},
month = {5},
url = {https://hdl.handle.net/10481/86531},
abstract = {Decidual stromal cells (DSCs) are the most abundant cellular component of human decidua and play a central
role in maternal–fetal immune tolerance. Antigen phenotyping and functional studies recently confirmed
the relationship of DSCs with mesenchymal stem/stromal cells (MSCs) and pericytes, the latter two
cell types being closely related or identical. The present study investigated the effect of decidualization, a
process of cell differentiation driven by progesterone (P4) and other pregnancy hormones, on the MSC/pericyte
characteristics of DSCs. To this end we isolated undifferentiated DSC (preDSC) lines that were decidualized
in vitro (dDSC) by the effect of P4 and cAMP. Using flow cytometry, we found significant downmodulation
of the expression of the MSC/pericyte markers α-smooth muscle actin, nestin, CD140b, CD146 and
SUSD2 in dDSCs. The dDSCs did not differ, compared to preDSCs, in the expression of angiogenic factors
(characteristic of pericytes) HGF, FGF2, ANGPT1 or VEGF according to RT-PCR results, but had significantly
increased PGF expression. In migration assays, preDSC-conditioned media had a chemotactic effect on the
THP-1 monocytic line (characteristic of pericytes), and this effect was significantly greater in dDSCconditioned
media. Media conditioned with dDSC, but not with preDSC, induced apoptosis in 4 out of 6 different
tumor cell lines (characteristic of MSCs) according to propidium iodide staining and flow cytometry
results. Our findings show that decidualization induces phenotypic and functional changes in the MSC/pericyte
properties of DSCs that may have a role in the normal development of pregnancy.},
organization = {This article contributes to COST Action CA17116 “International Network for Translating Research on Perinatal Derivatives into Therapeutic Approaches (SPRINT)”, supported by COST (European Cooperation in Science and Technology).},
publisher = {Elsevier Ltd.},
keywords = {Angiogenic factors},
keywords = {Decidual stroma l cells},
keywords = {Decidualization},
keywords = {Pericytes},
keywords = {Pregnancy},
keywords = {Mesenchymal stem/stromal cells},
title = {Decidualization modulates the mesenchymal stromal/stem cell and pericyte characteristics of human decidual stromal cells. Effects on antigen expression, chemotactic activity on monocytes and antitumoral activity},
doi = {10.1016/j.jri.2021.103326},
author = {Ruiz Magaña, María José and Martínez Aguilar, Rocío and Llorca, Tatiana and Abadía-Molina, Ana C. and Ruiz Ruiz, Carmen and García Olivares, Enrique},
}