@misc{10481/86514,
year = {2018},
month = {1},
url = {https://hdl.handle.net/10481/86514},
abstract = {Introduction: Human decidual stromal cells (DSCs) play a key role in maternalefetal interactions. Precursors of DSCs (preDSCs) localize around vessels in both the endometrium and decidua. Previous studies suggested a relationship between preDSCs and pericytes because these cells share a perivascular location, alpha smooth muscle actin (a-SM actin) expression and the ability to contract under the effects of cytokines.
Methods: To further study this relationship, we established 15 human preDSC lines and 3 preDSC clones. The preDSC lines and clones were tested by flow cytometry with a panel of 29 monoclonal antibodies, 14 of which are pericyte markers. The expression of angiogenic factors was determined by RT-PCR, chemotactic activity was studied with the migration assay, and cell contractility was evaluated with the collagen cell contraction assay. Confocal microscopy was used to study decidual sections.
Results: Under the effect of progesterone and cAMP, these lines decidualized in vitro: the cells became rounder and secreted prolactin, a marker of physiological DSC differentiation (decidualization). The antigen phenotype of these preDSC lines and clones was fully compatible with that reported for pericytes. PreDSC lines displayed pericyte characteristics: they expressed angiogenic factors and showed chemotactic and cytokine-induced contractile activity. Confocal microscopic examination of decidual
sections revealed the expression of antigens detected in preDSC lines: a-SM actin colocalized with CD146, CD140b, MFG-E8, nestin, and STRO-1 (all of which are pericyte markers) in cells located around the vessels, a distinctive location of preDSCs and pericytes.
Discussion: Taken together, our results show that preDSCs are pericyte-like cells.},
organization = {This work was supported by the Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016, ISCIII-Subdirección General de Evaluación y Fomento de la Investigación, Ministerio de Economía y Competitividad, Spain (Grant PI16/01642), by ERDF/FEDER funding, European Community, by the basic sciences program of the Fondo Nacional para el financiamiento de la Ciencia, la Tecnología y la Innovación Francisco José de Caldas (COLCIENCIAS), and by Cal 658-2014, Basic Sciences Program, Colombia.},
publisher = {Elsevier Ltd.},
keywords = {Cytokines},
keywords = {Decidua},
keywords = {Decidual stromal cells},
keywords = {Natural killer cells},
keywords = {Pericytes},
title = {Human predecidual stromal cells have distinctive characteristics of pericytes: Cell contractility, chemotactic activity, and expression of pericyte markers and angiogenic factors},
doi = {10.1016/j.placenta.2017.11.010},
author = {Muñoz Fernández, Raquel and de la Mata, Claudia and Prados, Alejandro and Perea, Ana and Ruiz Magaña, María José and Llorca, Tatiana and Fernández-Rubio, Pablo and Blanco, Osmany and Abadía-Molina, Ana C. and García Olivares, Enrique},
}