@misc{10481/85978,
year = {2023},
month = {10},
url = {https://hdl.handle.net/10481/85978},
abstract = {The excessive accumulation of chloride (Cl−) in leaves due to salinity is frequently related to decreased yield in citrus. Two salt tolerance experiments to detect quantitative trait loci (QTLs) for leaf concentrations of Cl−, Na+, and other traits using the same reference progeny derived from the salt-tolerant Cleopatra mandarin (Citrus reshni) and the disease-resistant donor Poncirus trifoliata were performed with the aim to identify repeatable QTLs that regulate leaf Cl− (and/or Na+) exclusion across independent experiments in citrus, as well as potential candidate genes involved. A repeatable QTL controlling leaf Cl− was detected in chromosome 6 (LCl-6), where 23 potential candidate genes coding for transporters were identified using the C. clementina genome as reference. Transcriptomic analysis revealed two important candidate genes coding for a member of the nitrate transporter 1/peptide transporter family (NPF5.9) and a major facilitator superfamily (MFS) protein. Cell wall biosynthesis- and secondary metabolism-related processes appeared to play a significant role in differential gene expression in LCl-6. Six likely gene candidates were mapped in LCl-6, showing conserved synteny in C. reshni. In conclusion, markers to select beneficial Cleopatra mandarin alleles of likely candidate genes in LCl-6 to improve salt tolerance in citrus rootstock breeding programs are provided.},
organization = {Spanish Ministry of Science and Innovation/Spanish Research Agency RTA2011-00132-C2, AGL2014-56675-R, AGL2017-82452-C2, PID2021-124599OB-I00},
organization = {Generalitat Valenciana 51917},
organization = {European Regional Development Fund (ERDF)},
organization = {MCIN/AEI/10.13039/501100011033},
organization = {ERDF “A way of making Europe”},
organization = {CSIC grant (JAEINT_19_00566)},
publisher = {MDPI},
keywords = {QTL analysis},
keywords = {Citrus reshni},
keywords = {Poncirus trifoliata},
keywords = {Rootstock breeding},
keywords = {Yield},
keywords = {Cl− homeostasis},
keywords = {Root growth},
keywords = {Plasticity},
title = {Combining Genetic and Transcriptomic Approaches to Identify Transporter-Coding Genes as Likely Responsible for a Repeatable Salt Tolerance QTL in Citrus},
doi = {10.3390/ijms242115759},
author = {Asins, María José and Espinosa Rodríguez, Jesús and Traverso Gutiérrez, José Ángel and Belver Cano, Andrés},
}