@misc{10481/85403,
year = {2023},
month = {9},
url = {https://hdl.handle.net/10481/85403},
abstract = {To explore the connection between chloroplast and coffee resistance factors, designated as SH1 to SH9, whole genomic DNA of 42 coffee genotypes was sequenced, and entire chloroplast genomes were de novo assembled. The chloroplast phylogenetic haplotype network clustered individuals per species instead of SH factors. However, for the first time, it allowed the molecular validation of Coffea arabica as the maternal parent of the spontaneous hybrid “Híbrido de Timor”. Individual reads were also aligned on the C. arabica reference genome to relate SH factors with chloroplast metabolism, and an in-silico analysis of selected nuclear-encoded chloroplast proteins (132 proteins) was performed. The nuclear-encoded thioredoxin-like membrane protein HCF164 enabled the discrimination of individuals with and without the SH9 factor, due to specific DNA variants linked to chromosome 7c (from C. canephora-derived sub-genome). The absence of both the thioredoxin domain and redox-active disulphide center in the HCF164 protein, observed in SH9 individuals, raises the possibility of potential implications on redox regulation. For the first time, the identification of specific DNA variants of chloroplast proteins allows discriminating individuals according to the SH profile. This study introduces an unexplored strategy for identifying protein/genes associated with SH factors and candidate targets of H. vastatrix effectors, thereby creating new perspectives for coffee breeding programs.},
organization = {CoffeeRES
	CEECIND/00399/2017/CP1423/CT0004, PTDC/ASP-PLA/29779/2017},
organization = {University of Bristol by Oracle for Research},
organization = {Biotechnology and Biological Sciences Research Council
 BB/X009831/1, BBW003449/1	BBSRC},
organization = {University of Bristol},
organization = {Fundação para a Ciência e a Tecnologia
 LA/P/0140/2020, UID/AGR/04129/2020, UIDB/04378/2020, UIDP/04378/2020	FCT},
organization = {European Regional Development Fund
 PTDC/ASP-PLA/32429/2017	ERDF},
publisher = {Springer Nature},
title = {The chloroplast protein HCF164 is predicted to be associated with Coffea SH 9 resistance factor against Hemileia vastatrix},
doi = {10.1038/s41598-023-41950-4},
author = {Guerra‑Guimarães, Leonor and Mira‑Jover, Andrea and Muñoz Pajares, Antonio Jesús},
}