@misc{10481/85242,
year = {2022},
month = {12},
url = {https://hdl.handle.net/10481/85242},
abstract = {Mass cytometry (MC) is a powerful large-scale immune monitoring technology. To maximize MC data quality, we present a protocol for whole blood analysis together with an R package, Cyto Quality Pipeline (CytoQP), which minimizes the experimental artifacts and batch effects to ensure data reproducibility. We describe the steps to stimulate, fix, and freeze blood samples before acquisition to make them suitable for retrospective studies. We then detail the use of bar-coding and reference samples to facilitate multicenter and multi-batch experiments.For complete details on the use and execution of this protocol, please refer to Rybakowska et al. (2021a) and (2021b).},
organization = {IMI2-JU project GA
	831434},
organization = {IMI-JUproject GA
	115565},
organization = {European Molecular Biology Organization (EMBO)
	7966},
organization = {Junta de Andalucía
	EF-0091-2018},
organization = {Programa Nicolás Monardes
	C2-0002-2019},
organization = {European Union-NextGenerationEU},
organization = {Ministry of Universities (Spain's Government) and the Recovery, Transformation and Resilience Plan},
publisher = {Elsevier},
title = {Protocol for large scale whole blood immune monitoring by mass cytometry and Cyto Quality Pipeline},
doi = {10.1016/j.xpro.2022.101697},
author = {Rybakowska, Paulina and Martorell Marugán, Jordi and Alarcón Riquelme, Marta Eugenia and Marañón Lizana, Concepción},
}