@misc{10481/85221,
year = {2023},
month = {8},
url = {https://hdl.handle.net/10481/85221},
abstract = {In this work, Engineered Living Materials (ELMs), based on the combination of genetically-modified bacteria and
mineral-reinforced organic matrices, and endowed with self-healing or regenerative properties and adaptation to
specific biological environments were developed. Concretely, we produced ELMs combining human mesenchymal
stem cells (hMSCs) and Lactococcus lactis (L. lactis), which was specifically programmed to deliver bone
morphogenetic protein (BMP-2) upon external stimulation using nisin, into mineralized alginate matrices. The
hybrid organic/inorganic matrix was built through a protocol, inspired by bone mineralization, in which alginate
(Alg) assembly and apatite (HA) mineralization occurred simultaneously driven by calcium ions. Chemical
composition, structure and reologhical properties of the hybrid 3D matrices were dedicately optimized prior the
incorportation of the living entities. Then, the same protocol was reproduced in the presence of hMSC and
engineered L. lactis that secrete BMP-2 resulting in 3D hybrid living hydrogels. hMSC viability and osteogenic
differentiation in the absence and presence of the bacteria were evaluated by live/dead and quantitative realtime
polymerase chain reaction (qPCR) and immunofluorescence assays, respectively. Results demonstrate
that these 3D engineered living material support osteogenic differentiation of hMSCs due to the synergistic effect
between HA and the growth factors BMP-2 delivered by L. lactis},
organization = {EPSRC (EP/P001114/1)},
organization = {U.K. Regenerative Medicine Platform “Acellular/Smart
Materials-3D Architecture” (MR/R015651/1)},
organization = {Junta de Andalucía
through the collaborative project NanoFERTI (P18-TP-0969)},
organization = {The
Spanish MCIN/AEI/10.13039/501100011033},
organization = {The “European
Union” NextGenerationEU/PRTR (project number PDC2022-133191-
I00)},
organization = {EMBO Scientific
Exchange Grant (SEG number 9741)},
organization = {RYC2021-032734-I},
organization = {MCIN/AEI/10.13039/501100011033},
organization = {ESF Investing in your
future},
publisher = {Elsevier},
keywords = {Living materials},
keywords = {Engineered bacteria},
keywords = {Calcium phosphate},
keywords = {Biomineralization},
keywords = {Osteogénesis},
keywords = {Tissue engineering},
title = {Bioinspired mineralization of engineered living materials to promote osteogenic differentiation},
doi = {10.1016/j.bioadv.2023.213587},
author = {Parra Torrejón, Belén and Ramírez Rodríguez, Gloria Belén and Delgado López, José Manuel},
}