@misc{10481/85106,
year = {2023},
month = {1},
url = {https://hdl.handle.net/10481/85106},
abstract = {Aim

To assess and compare the microbiome of paired root apices and periapical lesions from cases with failed endodontic treatment and to associate the microbiome and bacterial metabolic pathways in both sites with asymptomatic apical periodontitis (AAP) and symptomatic apical periodontitis (SAP), using next-generation sequencing (NGS).
Methodology

Matched root apices and periapical lesions of patients with failed root canal treatments were surgically extracted. Specimens were cryopulverized, bacterial DNA was extracted and the V3–V4 hypervariable regions of the 16 S rRNA gene were amplified and sequenced using the Illumina Miseq platform. Diversity and community composition were studied in the paired samples, as well as in AAP and SAP cases. Diversity indices were compared in each case by means of the Wilcoxon matched-pairs signed rank and Mann–Whitney U tests. Differences in the community composition were explored with multivariate statistical analysis and Linear discriminant analysis Effect Size (LEfSe). Bacterial functional study was performed through the Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt) analysis.
Results

Twenty-one paired apices and lesions were successfully sequenced and analysed, identifying a total of 21 phyla and 600 genera. A higher alpha-diversity was observed in the periapical lesions, although no global differences in the community composition between the two sites were found (p = .87), the most prevalent genera being Fusobacterium, Porphyromonas and Streptococcus. Prevotella, Clostridiales_vadinBB60_group, Bosea, Phreatobacter, Afipia and Xanthobacteriaceae_unclassified were enriched in SAP samples, while Pseudopropionibacterium, Campylobacter and Peptoniphilus were significantly more abundant in AAP cases (p < .05). Metabolic pathways involved in the amino acid metabolism or degradation and flagellum assembly were more abundant in SAP samples, whereas glucose metabolism-related pathways were associated with AAP.
Conclusions

The bacterial community composition was similar in the apices and periapical lesions. The microbiome was different in AAP and SAP samples, gram-negative bacteria showing higher relative abundances in SAP cases. An association was observed between amino acid degradation and flagellum assembly pathways, and the development of tenderness to percussion or palpation.},
organization = {FEDER/Junta de
Andalucía- Consejería de Economía y Conocimiento
A1- CTS-174-UGR18},
organization = {Ministry
of Science and Innovation of Spain},
organization = {European Regional Development Funds  
PID2020-120481RB-I00},
organization = {University of Almería
  PPUENTE2021-006},
organization = {Ministry of
Universities, Spain  FPU20/03952},
publisher = {Wiley},
keywords = {Apices},
keywords = {Microbiome},
keywords = {Next-generation sequencing},
keywords = {Periapical lesions},
keywords = {Persistent apical periodontitis},
keywords = {Symptoms},
title = {Microbiome in paired root apices and periapical lesions and its association with clinical signs in persistent apical periodontitis using next-generation sequencing},
doi = {10.1111/iej.13893},
author = {Pérez Carrasco, Virginia and Uroz Torres, David and Soriano, Miguel and Solana, Carmen and Ruiz Linares, Matilde and García Salcedo, José Antonio and Arias Moliz, María Teresa},
}