@misc{10481/85014,
year = {2023},
month = {8},
url = {https://hdl.handle.net/10481/85014},
abstract = {Numerous lines of evidence confirm that decidual stromal cells
(DSCs) play a key role in maternal–fetal immune tolerance. Under the influence
of progesterone and other hormones, the DSCs go through a process of
differentiation (decidualization) during normal pregnancy. In mice, DSCs inhibit
the expression of chemokines that attract abortigenic Th1 and Tc cells to the
decidua. We have studied this phenomenon in humans.
Methods: We established human DSC lines and decidualized these cells in vitro
with progesterone and cAMP. We determined the expression of the chemokines
CXCL9, CXCL10 and CXCL11, whose receptor CXCR3 is expressed by Th1 and Tc
cells, in undifferentiated DSCs and decidualized DSCs by qRT-PCR. Activated
CD3+CXCR3+ cells, including CD4+ Th1 cells and CD8+ Tc cells, were induced
in vitro. The migration capacity of these activated lymphocytes was investigated
in Transwell chambers with conditioned media from undifferentiated and
decidualized DSCs.
Results: We demonstrated that CXCL9 was not expressed by DSCs, whereas the
expression of CXCL10 and CXCL11 was inhibited in decidualized cells. Conditioned
media from decidualized cells significantly inhibited the migration of Th1 and Tc
cells.Wefound that decidualized cells secrete factors ofMWless than 6000–8000
Da, which actively inhibit the chemotaxis of these lymphocytes.
Discussion: These results confirm in humans that decidualization of DSCs
inhibits the expression by these cells of chemokines that attract Th1 and Tc
cells and induces the secretion by DSCs of factors that inhibit the chemotaxis of
these lymphocytes, thus preventing the arrival of abortigenic T cells in
the decidua.},
organization = {Financial support was provided by Proyectos de I+D+I through the
Programa Operativo Feder Andalucı́a (Grant B-CTS-228-UGR20)},
publisher = {Frontiers},
keywords = {Chemotaxis},
keywords = {Decidual stromal cells},
keywords = {Decidualization},
keywords = {Th1 cells},
keywords = {Tc cells},
title = {Decidualized human decidual stromal cells inhibit chemotaxis of activated T cells: a potential mechanism of maternal-fetal immune tolerance},
doi = {10.3389/fimmu.2023.1223539},
author = {Llorca, Tatiana and Ruiz Magaña, María José and Aguilar Martínez, José Luis and García Valdeavero, Olga Marı´a and Rodríguez Doña, Lucia and Abadía Molina, Ana Clara and Ruiz Ruiz, María Carmen and García Olivares, Enrique Fernando},
}