@misc{10481/84875,
year = {2023},
month = {7},
url = {https://hdl.handle.net/10481/84875},
abstract = {An adverse intrauterine or periconceptional environment, such as hyperglycemia during pregnancy, can affect the
DNA methylation pattern both in mothers and their offspring. In this study, we explored the epigenetic profile in
maternal peripheral blood samples through pregnancy to find potential epigenetic biomarkers for gestational diabetes
mellitus (GDM), as well as candidate genes involved in GDM development. We performed an epigenome-wide
association study in maternal peripheral blood samples in 32 pregnant women (16 with GDM and 16 non-GDM) at
pregnancy week 24–28 and 36–38. Biochemical, anthropometric, and obstetrical variables were collected from all the
participants. The main results were validated in an independent cohort with different ethnic origin (European = 307;
South Asians = 165). Two hundred and seventy-two CpGs sites remained significantly different between GDM and
non-GDM pregnant women across two time points during pregnancy. The significant CpG sites were related to
pathways associated with type I diabetes mellitus, insulin resistance and secretion. Cg01459453 (SELP gene) was the
most differentiated in the GDM group versus non-GDM (73.6 vs. 60.9, p = 1.06E−11; FDR = 7.87E−06). Three CpG sites
(cg01459453, cg15329406, and cg04095097) were able to discriminate between GDM cases and controls (AUC = 1;
p = 1.26E−09). Three differentially methylated positions (DMPs) were replicated in an independent cohort. To conclude,
epigenetic marks during pregnancy differed between GDM cases and controls suggesting a role for these
genes in GDM development. Three CpGs were able to discriminate GDM and non-GDM groups with high specificity
and sensitivity, which may be biomarker candidates for diagnosis or prediction of GDM.},
organization = {Juan Rodes program from "Instituto de Salud Carlos III"	JR20-00040},
organization = {Miguel Servet Type I program from the ISCIII-Madrid, Spain	CP20/00066},
organization = {PFIS program	FI19/00178},
organization = {Ministerio de trabajo y economia social de Espana},
organization = {Garantia Juvenil program	POEJ-0039-18},
organization = {Junta de Andalucia	RC-0008-2021},
organization = {Centros de Investigacion Biome-dica en Red" (CIBER)},
organization = {Institute of Health Carlos III (ISCIII)	CB06/03/0018},
organization = {Spanish Government	PI18/01175
PI21/01864},
organization = {Servicio Andaluz de Salud,"	PI-0283-2018
PI-0419-2019},
organization = {European Union (EU)},
publisher = {Springer Nature},
keywords = {Gestational diabetes mellitus},
keywords = {DNA methylation},
keywords = {Epigenetics},
keywords = {Epigenome-wide association study},
keywords = {Diabetes},
keywords = {Pregnancy},
keywords = {Validation},
title = {Epigenetic marks associated with gestational diabetes mellitus across two time points during pregnancy},
doi = {10.1186/s13148-023-01523-8},
author = {Linares Pineda, Teresa María and Sánchez Pozo, Antonio},
}