@misc{10481/84657,
year = {2023},
month = {6},
url = {https://hdl.handle.net/10481/84657},
abstract = {The search for methanotrophs as plant-growth-promoting rhizobacteria (PGPR) presents
an important contribution to mitigating the impact of global warming by restoring the natural soil
potential for consuming methane while benefiting plants during droughts. Our in silico simulations
suggest that water, produced as a byproduct of methane oxidation, can satisfy the cell growth
requirement. In addition to water, methanotrophs can produce metabolites that stimulate plant
growth. Considering this, we proposed that applying methanotrophs as PGPR can alleviate the effect
of droughts on crops, while stimulating atmospheric methane consumption. In this work, we isolated
a series of methanotrophic communities from the rhizospheres of different crops, including Italian
sweet pepper and zucchini, using an atmosphere enriched with pure methane gas, to determine their
potential for alleviating drought stress in wheat plants. Subsequently, 23 strains of nonmethanotrophic
bacteria present in the methanotrophic communities were isolated and characterized. We then
analyzed the contribution of the methane-consuming consortia to the improvement of plant growth
under drought conditions, showing that some communities contributed to increases in the wheat
plants’ lengths and weights, with statistically significant differences according to ANOVA models.
Furthermore, we found that the presence of methane gas can further stimulate the plant–microbe
interactions, resulting in larger plants and higher drought tolerance},
organization = {Spanish Ministry for Economy and Competitiveness
within the context of the research project and the program Salvador de Madariaga grant number
PID2021-127623OB-I00)},
organization = {Spanish Ministry for Economy and Competitiveness
within the context of the research project and the program Salvador de Madariaga grant number
PID2021-127623OB-I00)},
organization = {Junta de Andalucía (grant P18-RT-976)},
organization = {DOE
DE-SC0019181},
publisher = {MDPI},
keywords = {Greenhouse gases},
keywords = {Methanotrophic communities},
keywords = {PGPR},
keywords = {Triticum aestivum},
keywords = {Water stress},
keywords = {Biostimulants},
title = {Isolation of Methane Enriched Bacterial Communities and Application as Wheat Biofertilizer under Drought Conditions: An Environmental Contribution},
doi = {10.3390/plants12132487},
author = {Barros Rodríguez, Adoración and García Gálvez, Carlos and Pacheco, Pamela and Kalyuzhnaya, Marina G. and Manzanera Ruiz, Maximino Enrique},
}