@misc{10481/83856,
year = {2023},
month = {7},
url = {https://hdl.handle.net/10481/83856},
abstract = {Background: Meniere Disease (MD) is an inner ear syndrome, characterized by episodes of vertigo, tinnitus and fluctuating sensorineural hearing loss. The pathological mechanism leading to sporadic MD is still poorly understood, however an allergic inflammatory response seems to be involved in some patients with MD. Objective: Decipher an immune signature associated with the syndrome. Methods: We performed mass cytometry immune profiling on peripheral blood from MD patients and controls. We analyzed differences in state and differences in abundance of the different cellular subsets. IgE levels were quantified through ELISA on supernatant of cultured whole blood. Results: We have identified two clusters of individuals according to the single cell cytokine profile. These clusters presented differences in IgE levels, immune cell population abundance, including a reduction of CD56dim NKcells, and changes in cytokine expression with a different response to bacterial and fungal antigens. Conclusion: Our results support a systemic inflammatory response in some MD patients that show a type 2 response with allergic phenotype, which could benefit from personalized IL-4 blockers.},
organization = {FEDER Funds
	B-CTS-68-UGR20,
	
	B-CTS-68-UGR20},
organization = {Instituto de Salud Carlos III
Spanish Government
	PI17/1644,
	
	PI20-1126,
	
	CD20/0153,
	
	848261},
organization = {EU},
organization = {Meniere's Society UK},
organization = {Impact Data Science
	F18/00228},
organization = {Horizon 2020
	IMP0001},
organization = {Universidad de Granada/CBUA},
publisher = {Elsevier},
keywords = {Allergy},
keywords = {Inflammation},
keywords = {Cytokines},
keywords = {Mass cytometry},
keywords = {Meniere Disease},
title = {Single-cell immune profiling of Meniere Disease patients},
doi = {10.1016/j.clim.2023.109632},
author = {Flook, Marisa and Escalera Balsera, Alba and Rybakowska, Paulina and Frejo, Lidia and Alarcón Riquelme, Marta Eugenia and Lopez-Escamez, Jose A.},
}