@misc{10481/82976, year = {1986}, month = {3}, url = {https://hdl.handle.net/10481/82976}, abstract = {Utilizando el sistema pH-stat, tecnica volumétrica-potenciométrica, se han determinado las actividades colinesterasas de eritrocitos y plasma de ganado vacuno y porcino. Al estimar la influencia del anticoagulante se concluye que EDTA y heparina son los anticoagulantes idóneos para posteriores estudios de dichos enzimas. Se establece en 37ºC y pH 8,0, la temperatura y pH óptimos de medida de actividad "in vitro" del enzima acetilcolinesterasa. A pH acidos se observa un descenso brusco de la actividad enzimática, haciendose nula a pH próximo a 5,0.}, abstract = {Using the pH-stat system, a volumetric-potenciometric technique, the chollnesterases activities of erythrocytes and serum from cattle and pig were studied. The analysis of different anticoagulants effect reveals that EDT A and heparin are the preferred anticoagulants for belong studies of those enzymes. A 370C temperature and pH=8,0 are the optimal values for "in vitro" acetylcholinesterase acti vity measurement. A t acidic pH a strong de crease of the enzyme activity is detected. The activity value is zero when the pH is about 5,0.}, publisher = {Universidad de Granada, Facultad de Farmacia.}, title = {Influencia del anticoagulante, temperatura y pH sobre la actividad colinesterasa de ganado vacuno y porcino}, author = {López López, M. C. and Hermoso, R. and Monteoliva, m and Thomas, L.}, }