@misc{10481/82749,
year = {2023},
month = {4},
url = {https://hdl.handle.net/10481/82749},
abstract = {Alkalinity is an important environmental factor that affects crop production and will be exacerbated in the
current climate change scenario. Thus, the presence of carbonates and high pH in soils negatively impacts
nutrient assimilation and photosynthesis and causes oxidative stress. A potential strategy to improve tolerance to
alkalinity could be the modification of cation exchanger (CAX) activity, given that these transporters are
involved in calcium (Ca2+) signaling under stresses. In this study, we used three Brassica rapa mutants (BraA.
cax1a-4, BraA.cax1a-7, and BraA.cax1a-12) from the parental line ‘R-o-18’ that were generated by Targeting
Induced Local Lesions in Genomes (TILLING) and grown under control and alkaline conditions. The objective
was to assess the tolerance of these mutants to alkalinity stress. Biomass, nutrient accumulation, oxidative stress,
and photosynthesis parameters were analyzed. The results showed that BraA.cax1a-7 mutation was negative for
alkalinity tolerance because it reduced plant biomass, increased oxidative stress, partially inhibited antioxidant
response, and lowered photosynthesis performance. Conversely, the BraA.cax1a-12 mutation increased plant
biomass and Ca2+ accumulation, reduced oxidative stress, and improved antioxidant response and photosynthesis
performance. Hence, this study identifies BraA.cax1a-12 as a useful CAX1 mutation to enhance the
tolerance of plants grown under alkaline conditions.},
organization = {Plan Propio de Investigación y Transferencia, University of Granada awarded},
publisher = {Elsevier},
keywords = {Brassica rapa},
keywords = {CaCO3},
keywords = {Mineral elements},
keywords = {Oxidative stress},
keywords = {Photosynthesis},
keywords = {TILLING},
title = {Evaluation of the alkalinity stress tolerance of three Brassica rapa CAX1 TILLING mutants},
doi = {10.1016/j.plaphy.2023.107712},
author = {Navarro León, Eloy and Atero Calvo, Santiago and Ríos Ruiz, Juan José and Blasco León, Begoña},
}