@misc{10481/82043,
year = {2023},
month = {1},
url = {https://hdl.handle.net/10481/82043},
abstract = {The potential of pluripotent cells to respond to developmental cues and trigger
cell differentiation is enhanced during the G1 phase of the cell cycle, but the
molecular mechanisms involved are poorly understood. Variations in polycomb
activity during interphase progression have been hypothesized to regulate the
cell-cycle-phase-dependent transcriptional activation of differentiation genes
during lineage transition in pluripotent cells. Here, we show that recruitment of
Polycomb Repressive Complex 1 (PRC1) and associated molecular functions,
ubiquitination of H2AK119 and three-dimensional chromatin interactions, are
enhanced during S and G2 phases compared to the G1 phase. In agreement with
the accumulation of PRC1 at target promoters upon G1 phase exit, cells in S and
G2 phases show firmer transcriptional repression of developmental regulator
genes that is drastically perturbed upon genetic ablation of the PRC1 catalytic
subunit RING1B. Importantly, depletion of RING1B during retinoic acid stimu-
lation interferes with the preference of mouse embryonic stem cells (mESCs) to
induce the transcriptional activation of differentiation genes in G1 phase. We
propose that incremental enrolment of polycomb repressive activity during
interphase progression reduces the tendency of cells to respond to develop-
mental cues during S and G2 phases, facilitating activation of cell differentiation
in the G1 phase of the pluripotent cell cycle.},
organization = {Ministry of Science and Innovation, Spain (MICINN)
Spanish Government
	PID2019-108108-100,
	
	EUR2021-122005},
organization = {Andalusian regional government
	PIER-0211-2019,
	
	PY20_00681},
organization = {University of Granada
	A-BIO-6-UGR20},
organization = {Wellcome Trust
	209400/Z/17/Z},
organization = {European Research Council (ERC)
European Commission
	862022},
organization = {Wellcome Trust
	PGC2018-095301-B-I00},
organization = {Ministry of Science and Innovation, Spain (MICINN)
Instituto de Salud Carlos III
Spanish Government},
organization = {European Commission
	RED2018-102553-T,
	
	H2020-MSCA-ITN-2019-860002},
organization = {European Commission
European Commission Joint Research Centre
	681440},
organization = {Agencia Española de Investigación},
organization = {110286/Z/15/Z},
publisher = {Springer Nature},
title = {Changes in PRC1 activity during interphase modulate lineage transition in pluripotent cells},
doi = {10.1038/s41467-023-35859-9},
author = {Gómez Asenjo, Helena and Alcázar Fabra, María and Espinosa-Martínez, Mencía and López Onieva, Lourdes and Gallardo de los Reyes, Amador and Martorell Marugán, Jordi and Carmona Sáez, Pedro and Sánchez Pozo, Antonio and Landeira Frías, David Marcos},
}