@misc{10481/81763,
year = {2023},
month = {3},
url = {https://hdl.handle.net/10481/81763},
abstract = {Development of an ideal biomaterial for clinical use is one of the main objectives of current
research in tissue engineering. Marine-origin polysaccharides, in particular agaroses, have been
widely explored as scaffolds for tissue engineering. We previously developed a biomaterial based on
a combination of agarose with fibrin, that was successfully translated to clinical practice. However,
in search of novel biomaterials with improved physical and biological properties, we have now
generated new fibrin-agarose (FA) biomaterials using 5 different types of agaroses at 4 different
concentrations. First, we evaluated the cytotoxic effects and the biomechanical properties of these
biomaterials. Then, each bioartificial tissue was grafted in vivo and histological, histochemical and
immunohistochemical analyses were performed after 30 days. Ex vivo evaluation showed high
biocompatibility and differences in their biomechanical properties. In vivo, FA tissues were biocompatible
at the systemic and local levels, and histological analyses showed that biointegration
was associated to a pro-regenerative process with M2-type CD206-positive macrophages. These
results confirm the biocompatibility of FA biomaterials and support their clinical use for the generation
of human tissues by tissue engineering, with the possibility of selecting specific agarose
types and concentrations for applications requiring precise biomechanical properties and in vivo
reabsorption times.},
organization = {Hispanagar SA, Burgos, Spain, through CDTI, Ministry of Science and Innovation, Spain, Programa Operativo Plurirregional de Crecimiento Inteligente (CRIN)	IDI-20180052},
organization = {Spanish Plan Nacional de Investigacion Cientifica, Desarrollo e Innovacion Tecnologica (I + D + I) of the Spanish Ministry of Science and Innovation (Instituto de Salud Carlos III)	FIS PI20/0317
FIS PI20/0318
FIS PI21/0980
ICI19/00024
ICI21/00010},
organization = {Junta de Andalucia	PE-0395-2019
PI-0442-2019
PI-0086-2020},
organization = {Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades	B-CTS-504-UGR20},
publisher = {MDPI},
keywords = {Tissue engineering},
keywords = {Biomaterials},
keywords = {Fibrin},
keywords = {Agarose},
keywords = {Biocompatibility},
title = {Fibrin and Marine-Derived Agaroses for the Generation of Human Bioartificial Tissues: An Ex Vivo and In Vivo Study},
doi = {10.3390/md21030187},
author = {Ortiz Arrabal, Olimpia and Irastorza Lorenzo, Ainhoa and Campos Sánchez, Fernando and Martín Piedra, Miguel Ángel and Carriel Araya, Víctor and Garzón Bello, Ingrid Johanna and Ávila-Fernández, Paula and Campos Muñoz, Antonio Jesús and Chato Astrain, Jesús and Alaminos Mingorance, Miguel},
}