@misc{10481/81566,
year = {2023},
month = {2},
url = {https://hdl.handle.net/10481/81566},
abstract = {Persistent infections have become a challenge in dentistry because of growing antibiotic
resistance. Nonsteroidal anti-inflammatory drugs (NSAIDs) appear to be a therapeutic alternative to
control biofilm infection. The objective of this work is to evaluate the antimicrobial activity and cytotoxicity
of sodium diclofenac (DCS), ibuprofen (IBP) and ibuprofen arginine (IBP-arginine) solutions
against endodontic polymicrobial biofilms. Sterile radicular dentin blocks of 4 mm   4 mm   0.7 mm
were used as substrate to grow biofilm. The dentin blocks were submerged into solutions for 5 min.
The antimicrobial activity was evaluated by means of the adenosine triphosphate (ATP) assay and
confocal laser scanning microscopy (CLSM). Fibroblasts 3T3-L1 (ECACC 86052701) were used to
test the cytotoxicity of irrigating solutions. The antibiofilm effects determined by the ATP assay
showed that 4% IBP-arginine solution exerted the highest antibiofilm activity, followed by 4% DCS
and 4% IBP, with statistical differences among groups (p < 0.001). As for CLSM, 4% DCS and 4%
IBP-arginine solutions gave the lowest viable cell percentages, without significant differences between
them. Cytotoxicity results at 1/10 dilution were similar for all solutions. At 1/100 dilution, a 4%
DCS solution obtained the lowest cell viability for both time periods assayed, 1 h and 24 h. The
IBP-arginine group showed the highest cell viability at 24 h. In this preliminary study, in terms of
antibiofilm activity and cytotoxicity, a mixed 4% IBP-arginine solution gave the most promising
results. NSAID solutions could be recommendable drugs for endodontic disinfection procedures},
organization = {Research Group CTS-167 of Junta de Andalucía, Spain.},
publisher = {MDPI},
keywords = {Antimicrobial activity},
keywords = {Endodontic biofilms},
keywords = {Diclofenac},
keywords = {Ibuprofen},
keywords = {Non-steroidal},
keywords = {Anti-inflammatory drugs},
title = {Antimicrobial Activity and Cytotoxicity of Nonsteroidal Anti-Inflammatory Drugs against Endodontic Biofilms},
doi = {10.3390/ antibiotics12030450},
author = {Ferrer Luque, Carmen María and Solana, Carmen and Aguado, Beatriz and Ruiz Linares, Matilde},
}