@misc{10481/81565, year = {2023}, month = {3}, url = {https://hdl.handle.net/10481/81565}, abstract = {This work studied the physical and oxidative stabilities of fish oil-in-water-in-olive oil double emulsions (O1/W/O2), where whey protein hydrolysate was used as a hydrophilic emulsifier. A 20 wt.% fish oil-in-water emulsion, stabilized with whey protein hydrolysate (oil: protein ratio of 5:2 w/w) and with a zeta potential of ~�����40 mV, only slightly increased its D4,3 value during storage at 8 C for seven days (from 0.725 to 0.897 m), although it showed severe physical destabilization when stored at 25 C for seven days (D4,3 value increased from 0.706 to 9.035 m). The oxidative stability of the 20 wt.% fish oil-in-water emulsion decreased when the storage temperature increased (25 vs. 8 C) as indicated by peroxide and p-anisidine values, both in the presence or not of prooxidants (Fe2+). Confocal microscopy images confirmed the formation of 20 wt.% fish oil-in-water-in-olive oil (ratio 25:75 w/w) using Polyglycerol polyricinoleate (PGPR, 4 wt.%). Double emulsions were fairly physically stable for 7 days (both at 25 and 8 C) (Turbiscan stability index, TSI < 4). Moreover, double emulsions had low peroxide (<7 meq O2/kg oil) and p-anisidine (<7) values that did not increase during storage independently of the storage temperature (8 or 25 C) and the presence or not of prooxidants (Fe2+), which denotes oxidative stability.}, organization = {the I+D+i projects CTQ2017-87076-R and PID2020-114137RBI00 funded by MCIN/AEI/10.13039/501100011033}, publisher = {MDPI}, keywords = {Double emulsions}, keywords = {Omega-3 polyunsaturated fatty acids}, keywords = {Protein hydrolysates}, keywords = {Lipid oxidation}, title = {Evaluation of the Physical and Oxidative Stabilities of Fish Oil-in-Water-in-Olive Oil Double Emulsions (O1/W/O2) Stabilized with Whey Protein Hydrolysate}, doi = {10.3390/antiox12030762}, author = {Padial Domínguez, Marta and García Moreno, Pedro Jesús and González-Beneded, Rubén and Guadix Escobar, Antonio María and Guadix Escobar, Emilia María}, }