@misc{10481/80937,
year = {2023},
month = {2},
url = {https://hdl.handle.net/10481/80937},
abstract = {In recent years, the consumption of blue-green algae (BGA) dietary supplements is increasing because of their health benefits. However, cyanobacteria can produce cyanotoxins, which present serious health risks. In this work we propose hydrophilic interaction liquid chromatography coupled with tandem mass spectrometry (HILIC-MS/MS) to determine cyanotoxins in BGA dietary supplements. Target toxins, including microcystin-leucine-arginine (MC-LR) and microcystin-arginine-arginine (MC-RR), nodularin, anatoxin-a and three non-protein amino acids, beta-N-methylamino-L-alanine (BMAA), 2,4-diaminobutyric acid (DAB) and N-(2-aminoethyl)glycine (AEG), were separated using a SeQuant ZIC-HILIC column. Cyanotoxin extraction was based on solid-liquid extraction (SLE) followed by a tandem-solid phase extraction (SPE) procedure using Strata-X and mixed-mode cation-exchange (MCX) cartridges. The method was validated for BGA dietary supplements obtaining quantification limits from 60 to 300 mu g center dot kg(-1). Nine different commercial supplements were analyzed, and DAB, AEG, and MCs were found in some samples, highlighting the relevance of monitoring these substances as precaution measures for the safe consumption of these products.},
organization = {MCIN/ AEI	RTI2018-097043-B-I00},
organization = {Project Junta de Andalucia-Programa Operativo FEDER	B-AGR-202-UGR20},
organization = {MCIN/AEI and FSE	FPU17/03810},
publisher = {MDPI},
keywords = {Blue-green algae},
keywords = {Cyanotoxins},
keywords = {Dietary supplements},
keywords = {HILIC-MS/MS},
keywords = {Tandem-solid phase extraction},
title = {Determination of Multiclass Cyanotoxins in Blue-Green Algae (BGA) Dietary Supplements Using Hydrophilic Interaction Liquid Chromatography-Tandem Mass Spectrometry},
doi = {10.3390/toxins15020127},
author = {Aparicio Muriana, María del Mar and Lara Vargas, Francisco Jesús and Olmo Iruela, María Monsalud Del and García Campaña, Ana María},
}