@misc{10481/80864,
year = {2023},
month = {2},
url = {https://hdl.handle.net/10481/80864},
abstract = {Wharton’s jelly stem cells (WJSC) from the human umbilical cord (UC) are one of the most
promising mesenchymal stem cells (MSC) in tissue engineering (TE) and advanced therapies. The
cell niche is a key element for both, MSC and fully differentiated tissues, to preserve their unique
features. The basement membrane (BM) is an essential structure during embryonic development and
in adult tissues. Epithelial BMs are well-known, but similar structures are present in other histological
structures, such as in peripheral nerve fibers, myocytes or chondrocytes. Previous studies suggest the
expression of some BM molecules within the Wharton’s Jelly (WJ) of UC, but the distribution pattern
and full expression profile of these molecules have not been yet elucidated. In this sense, the aim of
this histological study was to evaluate the expression of main BM molecules within the WJ, cultured
WJSC and during WJSC microtissue (WJSC-MT) formation process. Results confirmed the presence
of a pericellular matrix composed by the main BM molecules—collagens (IV, VII), HSPG2, agrin,
laminin and nidogen—around the WJSC within UC. Additionally, ex vivo studies demonstrated the
synthesis of these BM molecules, except agrin, especially during WJSC-MT formation process. The
WJSC capability to synthesize main BM molecules could offer new alternatives for the generation of
biomimetic-engineered substitutes where these molecules are particularly needed.},
organization = {Instituto de Salud Carlos III},
organization = {Ministry of Science and Innovation, Spain (MICINN)
Spanish Government	FIS PI21/0980
FIS PI20/0317
FIS PI20/0318},
organization = {European Commission},
organization = {Junta de Andalucia	PE-0395-2019},
organization = {Universidad de Granada	B-CTS-450-UGR20
A-CTS-498-UGR18},
organization = {Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades Junta de Andalucia and European Union},
organization = {Junta de Andalucia	P18-RT-5059},
publisher = {MDPI},
keywords = {Umbilical cord (UC)},
keywords = {Wharton jelly stem cells (WJSC)},
keywords = {Extracellular matrix (ECM)},
keywords = {Basement membrane (BM)},
keywords = {Basal lamina (BL) stem cell-based microtissues (MT)},
keywords = {Tissue engineering (TE)},
title = {Expression of Basement Membrane Molecules by Wharton Jelly Stem Cells (WJSC) in Full-Term Human Umbilical Cords, Cell Cultures and Microtissues},
doi = {10.3390/cells12040629},
author = {Sánchez Porras, David and Durand Herrera, Daniel and Carmona Martos, Ramón and Blanco Elices, Cristina and Garzón Bello, Ingrid Johanna and Alaminos Mingorance, Miguel and García García, Óscar Darío and Chato Astrain, Jesús and Carriel Araya, Víctor},
}