@misc{10481/80860,
year = {2023},
month = {2},
url = {https://hdl.handle.net/10481/80860},
abstract = {Here, we propose a microfluidic paper-based analytical device (mu PAD) implemented with a near-field communication (NFC) tag as a portable, simple and fast colorimetric method for glutathione (GSH) determination. The proposed method was based on the fact that Ag+ could oxidize 3,3';,5,5'-tetramethylbenzidine (TMB) into oxidized blue TMB. Thus, the presence of GSH could cause the reduction of oxidized TMB, which resulted in a blue color fading. Based on this finding, we developed a method for the colorimetric determination of GSH using a smartphone. A mu PAD implemented with the NFC tag allowed the harvesting of energy from a smartphone to activate the LED that allows the capture of a photograph of the mu PAD by the smartphone. The integration between electronic interfaces into the hardware of digital image capture served as a means for quantitation. Importantly, this new method shows a low detection limit of 1.0 mu M. Therefore, the most important features of this non-enzymatic method are high sensitivity and a simple, fast, portable and low-cost determination of GSH in just 20 min using a colorimetric signal.},
organization = {BBVA Foundation},
organization = {European Commission
European Commission Joint Research Centre	H2020-MSCA-IF-2017-794885-SELFSEN},
organization = {Spanish Government	PID2019-103938RB-I00
PID2020-117344RBI00},
organization = {Junta de Andalucia	BFQM-243-UGR18
P18-RT-2961},
organization = {European Commission},
publisher = {MDPI},
keywords = {muPAD},
keywords = {NFC tag},
keywords = {Smartphone},
keywords = {Colorimetric assay},
keywords = {Glutathione determination},
keywords = {Health prognosis},
title = {Near-Field Communication Tag for Colorimetric Glutathione Determination with a Paper-Based Microfluidic Device},
doi = {10.3390/bios13020267},
author = {Ortiz Gómez, Inmaculada and Rivadeneyra Torres, Almudena and Salmerón, José F. and Orbe Payá, Ignacio De and Morales Santos, Diego Pedro and Capitán Vallvey, Luis Fermín and Salinas Castillo, Alfonso},
}