@misc{10481/80796,
year = {2023},
month = {2},
url = {https://hdl.handle.net/10481/80796},
abstract = {DPP IV, otherwise known as CD26 lymphocyte T surface antigen, is a
transmembrane glycoprotein also found in circulation in the blood. It plays an important
role in several processes like glucose metabolism and T-cell stimulation. Moreover, it is
overexpressed in renal, colon, prostate, and thyroid human carcinoma tissues. It can also
serve as a diagnostic in patients with lysosomal storage diseases. The biological and clinical
importance of having readouts for the activity of this enzyme, in physiological and disease
conditions, has led us to design a near-infrared (NIR) fluorimetric probe that also has the
characteristics of being ratiometric and excitable by two simultaneous NIR photons. The
probe consists of assembling an enzyme recognition group (Gly-Pro) (Mentlein, 1999;
Klemann et al., 2016) on the two-photon (TP) fluorophore (derivative of dicyanomethylene-
4H-pyran, DCM-NH2) disturbing its NIR characteristic internal charge transfer
(ICT) emission spectrum. When the dipeptide group is released by the DPP IV-specific
enzymatic action, the donor−acceptor DCM-NH2 is restored, forming a system that shows
high ratiometric fluorescence output. With this new probe, we have been able to detect, quickly and efficiently, the enzymatic activity
of DPP IV in living cells, human tissues, and whole organisms, using zebrafish. In addition, due to the possibility of being excited by
two photons, we can avoid the autofluorescence and subsequent photobleaching that the raw plasma has when it is excited by visible
light, achieving detection of the activity of DPP IV in that medium without interference.},
organization = {FEDER/Junta de Andaluc?a- Consejer?a de Transformaci?n Econ?mica, Industria, Con- ocimiento y Universidades/Proyecto A-FQM-230-UGR20	PID2020-114256RB-I00},
organization = {Spanish Government	FPU17/04749},
publisher = {American Chemical Society},
keywords = {DPP IV},
keywords = {Ratiometric fluorescent sensor},
keywords = {NIR probe},
keywords = {Two-photon excitation},
keywords = {Bioimaging},
title = {Ratiometric Two-Photon Near-Infrared Probe to Detect DPP IV in Human Plasma, Living Cells, Human Tissues, and Whole Organisms Using Zebrafish},
doi = {10.1021/acssensors.2c02025},
author = {Valverde Pozo, Javier and Paredes Martínez, José Manuel and Widmann, Thomas J. and Griñán Lisón, Carmen and García Rubiño, María Eugenia and Marchal Corrales, Juan Antonio and Álvarez Pez, José María and Talavera Rodríguez, Eva María},
}