@misc{10481/79047,
year = {2022},
month = {11},
url = {https://hdl.handle.net/10481/79047},
abstract = {Breast cancer is the most common type of cancer in women, with chemotherapy being
the main strategy. However, its effectiveness is reduced by drug resistance mechanisms. miR-21 is
upregulated in breast cancer that has been linked to drug resistance and carcinogenic processes. Our
aim was to capture miR-21 with a circular sponge (Circ-21) and thus inhibit the carcinogenic processes
and drug resistance mechanisms in which it participates. Proliferation, migration, colony formation,
cell cycle, and poly [ADP-ribose] polymerase 1 (PARP-1) and vascular endothelial growth factor
(VEGF) detection assays were performed with MCF7 breast cancer cells and MCF10A non-tumor
cells. In addition, doxorubicin resistance tests and detection of drug resistance gene expression were
performed in MCF7 cells. Reduction in proliferation, as well as migration and colony formation,
increased PARP-1 expression, inhibition of VEGF expression and cell cycle arrest in G2/M phase
were displayed in the Circ-21 MCF7, which were not observed in the MCF10A cells. Furthermore,
in the MCF7 cells, the Circ-21 enhanced the antitumor activity of doxorubicin and decreased the
expression of resistance genes: ABCA1, ABCC4, and ABCC5. Based on these results, the use of Circ-21
can be considered a first step for the establishment of an effective gene therapy in the treatment of
breast cancer.},
organization = {CTS-107 Group},
organization = {Instituto de Salud Carlos III},
organization = {European Commission		
PI19/01478},
publisher = {MDPI},
keywords = {miRNA},
keywords = {Circular sponges},
keywords = {miR-21},
keywords = {Doxorubicin},
keywords = {Breast cancer},
title = {Circular Sponge against miR-21 Enhances the Antitumor Activity of Doxorubicin against Breast Cancer Cells},
doi = {10.3390/ijms232314803},
author = {Rama, Ana R. and Lara, Patricia and Mesas Hernández, Cristina and Quiñonero Muñoz, Francisco José and Vélez Fernández, María Celia and Melguizo Alonso, Consolación and Prados Salazar, José Carlos},
}