@misc{10481/79026,
year = {2022},
month = {11},
url = {https://hdl.handle.net/10481/79026},
abstract = {Purpose: Lack of diagnostic and prognostic biomarkers in hepatocellular
carcinoma impedes stratifying patients based on their risk of developing
cancer. The aim of this study was to evaluate phenotypic and genetic
heterogeneity of circulating epithelial cells (CECs) based on
asialoglycoprotein receptor 1 (ASGR1) and miR-122-5p expression as
potential diagnostic and prognostic tools in patients with hepatocellular
carcinoma (HCC) and liver cirrhosis (LC).
Methods: Peripheral blood samples were extracted from LC and HCC patients
at different disease stages. CECs were isolated using positive immunomagnetic
selection. Genetic and phenotypic characterization was validated by double
immunocytochemistry for cytokeratin (CK) and ASGR1 or by in situ hybridization
with miR-122-5p and CECs were visualized by confocal microscopy.
Results: The presence of CECs increased HCC risk by 2.58-fold, however, this
was only significant for patients with previous LC (p = 0.028) and not for those
without prior LC (p = 0.23). Furthermore, the number of CECs lacking
ASGR1 expression correlated significantly with HCC incidence and absence
of miR-122-5p expression (p = 0.014; r = 0.23). Finally, overall survival was
significantly greater for patients at earlier cancer stages (p = 0.018), but this difference was only maintained in the group with the presence of CECs (p =
0.021) whereas progression-free survival was influenced by the absence of
ASGR1 expression.
Conclusion: Identification and characterization of CECs by ASGR1 and/or miR-
122-5p expression may be used as a risk-stratification tool in LC patients, as it
was shown to be an independent prognostic and risk-stratification marker in LC
and early disease stage HCC patients.},
organization = {Regional Ministry of Health of Andalusia},
organization = {Ministry of Economy, Competitiveness, Enterprises and Universities	PC-0522-2016
PC-0267-2017
PC-0033-2017},
organization = {Health Institute Carlos III (ISCIII)	DOC_01682
CD18/00126},
publisher = {Frontiers},
keywords = {Hepatocellular carcinoma},
keywords = {Liver cirrhosis},
keywords = {Circulating tumor cells},
keywords = {Precision medicine},
keywords = {Cancer interception},
title = {Hepatocellular carcinoma risk-stratification based on ASGR1 in circulating epithelial cells for cancer interception},
doi = {10.3389/fmolb.2022.1074277},
author = {Roa Colomo, Amparo and López Garrido, María Ángeles and Molina Vallejo, María Pilar and González Sánchez, Mercedes and Aranda García, Violeta and Salmerón Escobar, Francisco Javier and Lorente Acosta, José Antonio and Serrano Fernández, María José and Garrido Navas, María del Carmen},
}