@misc{10481/77617,
year = {2022},
month = {6},
url = {https://hdl.handle.net/10481/77617},
abstract = {The importance of egg natural defences to prevent bacterial contamination and their relation with hen age in
extended production cycles were evaluated. Egg-white from eggs of different hen age groups (up 100-weeks-old)
and lines (Hy-Line white and brown) were inoculated with Gram-positive Staphylococcus aureus or Gram-negative
Salmonella Typhimurium, ranging from 103-106 CFU/mL. Our results show that concentrations of egg-white
lysozyme and, particularly, ovotransferrin are important to modulate bacterial survival in a dose-dependent
matter. Depending on protein concentration, their effect ranges from bactericidal to bacteriostatic, with a
threshold for bacterial contamination that depends also on hen age and line. The concentrations of lysozyme and
ovotransferrin increased with hen age (up to 2 and 22 w/w% of total protein, respectively), and eggs laid by
older hens exhibited the greatest potential to prevent the growth of the highest Salmonella inoculum (106 CFU/
mL). Salmonella-penetration experiments demonstrated that non-contaminated eggs display significantly higher
concentrations of antimicrobial proteins. However, eggs from older hens needed a higher concentration of these
proteins (>20% ovotransferrin) to prevent bacterial contamination, showing that antimicrobial protein concentrations
in egg-whites was not the only factor influencing bacterial contamination. Finally, this study
demonstrated that egg-white of eggs produced by old hens are less prone to contamination by Salmonella.},
organization = {Spanish Government	CGL 2015-64683-P},
organization = {Premio de Investigacion 2019 (Instituto del Huevo)},
organization = {Junta de Andalucia	RNM-938},
organization = {Natural Sciences and Engineering Research Council of Canada (NSERC)	RGPIN2022-04410},
publisher = {Elsevier},
keywords = {Egg white},
keywords = {Hen},
keywords = {Antimicrobial proteins},
keywords = {Bacterial contamination},
keywords = {UPLC-Mass spectroscopy},
title = {Antimicrobial defenses of table eggs: Importance of antibacterial proteins in egg white as a function of hen age in an extended production cycle},
doi = {10.1016/j.fm.2022.104068},
author = {Jabalera Ruz, Ylenia María and Domínguez Gasca, Nazaret and Muñoz, Arantxa and Jiménez López, Concepción and Rodríguez Navarro, Alejandro},
}