@misc{10481/77343,
year = {2022},
month = {9},
url = {https://hdl.handle.net/10481/77343},
abstract = {Previous works have described the activity of Bifidobacterium longum subsp. infantis CECT
7210 (also commercially named B. infantis IM-1®) against rotavirus in mice and intestinal pathogens in
piglets, as well as its diarrhea-reducing effect on healthy term infants. In the present work, we focused
on the intestinal immunomodulatory effects of B. infantis IM-1® and for this purpose we used the
epithelial cell line isolated from colorectal adenocarcinoma Caco-2 and a co-culture system of human
dendritic cells (DCs) from peripheral blood together with Caco-2 cells. Single Caco-2 cultures and
Caco-2: DC co-cultures were incubated with B. infantis IM-1® or its supernatant either in the presence
or absence of Escherichia coli CECT 515. The B. infantis IM-1® supernatant exerted a protective effect
against the cytotoxicity caused by Escherichia coli CECT 515 on single cultures of Caco-2 cells as
viability reached the values of untreated cells. B. infantis IM-1® and its supernatant also decreased the
secretion of pro-inflammatory cytokines by Caco-2 cells and the co-cultures incubated in the presence
of E. coli CECT 515, with the response being more modest in the latter, which suggests that DCs
modulate the activity of Caco-2 cells. Overall, the results obtained point to the immunomodulatory
activity of this probiotic strain, which might underlie its previously reported beneficial effects.},
organization = {Laboratorios Ordesa, S. L.},
organization = {Center for the Development of Industrial Technology (CDTI) of the Spanish Ministry of Science and Innovation (TOLERA project)	20170873},
publisher = {MDPI},
keywords = {B. infantis IM-1®},
keywords = {Bifidobacterium longum subsp. infantis CECT 7210},
keywords = {Caco-2 cells},
keywords = {Co-cultures},
keywords = {Cytokines},
keywords = {Dendritic cells},
keywords = {Escherichia coli},
keywords = {Probiotics},
title = {Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515},
doi = {10.3390/ijms231810813},
author = {Álvarez Mercado, Ana Isabel and Plaza Díaz, Julio and Gil Hernández, Ángel and Fontana Gallego, Luis},
}