@misc{10481/76640,
year = {2022},
month = {6},
url = {http://hdl.handle.net/10481/76640},
abstract = {Emerging and unregulated contaminants end up in soils via stabilized/composted sewage
sludges, paired with possible risks associated with the development of microbial resistance to
antimicrobial agents or an imbalance in the microbial communities. An enrichment experiment
was performed, fortifying the sewage sludge with carbamazepine, ketoprofen and diclofenac as
model compounds, with the aim to obtain strains with the capability to transform these pollutants.
Culturable microorganisms were obtained at the end of the experiment. Among fungi, Cladosporium
cladosporioides, Alternaria alternata and Penicillium raistrickii showed remarkable degradation rates.
Population shifts in bacterial and fungal communities were also studied during the selective pressure
using Illumina MiSeq. These analyses showed a predominance of Ascomycota (Dothideomycetes
and Aspergillaceae) and Actinobacteria and Proteobacteria, suggesting the possibility of selecting native
microorganisms to carry out bioremediation processes using tailored techniques.},
organization = {Spanish Government},
organization = {European Research Founds	CTM2017-84332-R},
organization = {Junta de Andalucia	B-RNM-204-UGR20},
organization = {Consejo Nacional de Ciencia y Tecnologia (CONACyT)	377965	
739637},
organization = {Spanish Ministry of Universities (Maria Zambrano Program) - Next Generation EU (NGEU)},
publisher = {MDPI},
keywords = {Sewage sludge},
keywords = {Emerging pollutants},
keywords = {Selective pressure},
keywords = {Microbial communities},
keywords = {illumina MiSeq},
title = {High-Throughput Microbial Community Analyses to Establish a Natural Fungal and Bacterial Consortium from Sewage Sludge Enriched with Three Pharmaceutical Compounds},
doi = {10.3390/jof8070668},
author = {Ledezma Villanueva, Alejandro and Robledo Mahón, Tatiana and Ángeles de Paz, Gabriela and Pozo Llorente, Clementina and Manzanera Ruiz, Maximino Enrique and Calvo Sáinz, Concepción and Aranda Ballesteros, Elisabet},
}