@misc{10481/76621, year = {2022}, month = {7}, url = {http://hdl.handle.net/10481/76621}, abstract = {The most recent generation of bioengineered human skin allows for the efficient treatment of patients with severe skin defects. Despite UV sunlight can seriously affect human skin, the optical behavior in the UV range of skin models is still unexplored. In the present study, absorbance and transmittance of the UGRSKIN bioartificial skin substitute generated with human skin cells combined with fibrin-agarose biomaterials were evaluated for: UV-C (200–280 nm), -B (280–315 nm), and -A (315–400 nm) spectral range after 7, 14, 21 and 28 days of ex vivo development. The epidermis of the bioartificial skin substitute was able to mature and differentiate in a time-dependent manner, expressing relevant molecules able to absorb most of the incoming UV radiation. Absorbance spectral behavior of the skin substitutes showed similar patterns to control native skin (VAF > 99.4%), with values 0.85–0.90 times lower than control values at 7 and 14- days and 1.05–1.10 times the control values at 21- and 28-days. UV absorbance increased, and UV transmission decreased with culture time, and comparable results to the control were found at 21 and 28 days. These findings support the use of samples corresponding to 21 or 28 days of development for clinical purposes due to their higher histological similarities with native skin, but also because of their absorbance of UV radiation.}, organization = {Junta de Andalucia PE-0395-2019}, organization = {University of Granada B-CTS-450-UGR20 A.TEP.280.UGR18}, organization = {Consejeria de Transformacion Economica, Industria, Conocimiento y Universidades B-CTS-450-UGR20}, organization = {European Regional Development Fund (ERDF) through the "Una manera de hacer Europa" program Junta de Andalucia P20-00200}, organization = {Spanish Government PGC2018-101904-A-100}, publisher = {MDPI}, keywords = {Absorption}, keywords = {UV radiation}, keywords = {Bioengineered skin}, keywords = {Fibrin-agarose biomaterial}, title = {Optical Behavior of Human Skin Substitutes: Absorbance in the 200–400 nm UV Range}, doi = {10.3390/biomedicines10071640}, author = {Ruiz López, Javier and Cardona Pérez, Juan De La Cruz and Garzón Bello, Ingrid Johanna and Pérez Gómez, María Del Mar and Alaminos Mingorance, Miguel and Chato Astrain, Jesús and Ionescu, Ana María Andreea}, }