@misc{10481/76498,
year = {2021},
month = {6},
url = {http://hdl.handle.net/10481/76498},
abstract = {The role of leptin in the development of intestinal inflammation remains controversial, since proinflammatory and anti-inflammatory
effects have been described. This study describes the effect of the absence of leptin signaling in intestinal inflammation.
Experimental colitis was induced by intrarectal administration of trinitrobenzene sulfonic acid (TNBS) to lean and obese Zucker
rats (n = 10). Effects on inflammation and mucosal barrier were studied. Bacterial translocation and LPS concentration were evaluated
together with colonic permeability to 4-kDa FITC-dextran. Obese Zucker rats showed a lower intestinal myeloperoxidase
and alkaline phosphatase activity, reduced alkaline phosphatase sensitivity to levamisole, and diminished colonic expression of
Nos2, Tnf, and Il6, indicating attenuated intestinal inflammation, associated with attenuated STAT3, AKT, and ERK signaling in
the colonic tissue. S100a8 and Cxcl1 mRNA levels were maintained, suggesting that in the absence of leptin signaling neutrophil
activation rather than infiltration is hampered. Despite the lower inflammatory response, leptin resistance enhanced intestinal
permeability, reflecting an increased epithelial damage. This was shown by augmented LPS presence in the portal vein of colitic
obese Zucker rats, associated with induction of tissue nonspecific alkaline phosphatase, LPS-binding protein, and CD14 hepatic
expression (involved in LPS handling). This was linked to decreased ZO-1 immunoreactivity in tight junctions and lower occludin
expression. Our results indicate that obese Zucker rats present an attenuated inflammatory response to TNBS, but increased intestinal epithelial damage allowing the passage of bacterial antigens.},
organization = {Ministerio de Economía y Competitividad and the Fondo Europeo de Desarrollo Regional FEDER (SAF2011-22922, SAF2011-22812, BFU2014- 57736-P, and AGL2014-58883-R)},
organization = {Junta de Andalucía (CTS164, CTS235, and CTS6736)},
organization = {Ministerio de Educación},
keywords = {Intestinal barrier function},
keywords = {Leptin},
keywords = {Obesity},
title = {Leptin-resistant Zucker rats with trinitrobenzene sulfonic acid colitis present a reduced inflammatory response but enhanced epithelial damage},
doi = {doi:10.1152/ajpgi.00367.2020},
author = {Rivero Gutiérrez, Belén and Arredondo Amador, María and Gámez Belmonte, María de los Reyes and Sánchez De Medina López-Huertas, Fermín and Martínez Augustín, María Olga},
}