@misc{10481/76369,
year = {2022},
month = {6},
url = {http://hdl.handle.net/10481/76369},
abstract = {A strictly aerobic, chemoheterotrophic, endospore-forming, Gram-positive, rod-shaped
bacterial strain N3T was isolated from the feces of a river otter in Castril (Granada,
southern Spain). It is halotolerant, motile, and catalase-, oxidase-, ACC deaminase-,
and C4- and C8-lipase-positive. It promotes tomato plant growth and can reduce
virulence in Erwinia amylovora CECT 222T and Dickeya solani LMG 25993T through
interference in their quorum-sensing systems, although other antagonistic mechanisms
could also occur. A phylogenetic analysis of the 16S rRNA gene sequence as well as the
phenotypic and phylogenomic analyses indicated that the strain N3T is a novel species
of the genus Peribacillus, with the highest 16S rRNA sequence similar to that of Bacillus
frigoritolerans DSM 8801T (99.93%) and Peribacillus simplex DSM 1321T (99.80%).
Genomic digital DNA–DNA hybridization (dDDH) between the strain N3T and Bacillus
frigoritolerans DSM 8801T and Peribacillus simplex was 12.8 and 69.1%, respectively,
and the average nucleotide identity (ANIb) of strain N3T and Bacillus frigoritolerans
DSM 8801T and Peribacillus simplex was 67.84 and 93.21%, respectively. The genomic
G + C content was 40.3 mol%. Its main cellular fatty acids were anteiso-C15 : 0 and
iso-C15 : 0. Using 16S rRNA phylogenetic and in silico phylogenomic analyses, together
with the chemotaxonomic and phenotypic data, we demonstrated that the type strain
N3T (=CECT 30509T = LMG 32505T) is a novel species of the genus Peribacillus and
the name Peribacillus castrilensis sp. nov. is proposed.},
organization = {Spanish Ministerio
de Educación y Ciencia (AGL-2015-68806-R)},
organization = {Spanish Ministry of the Economy and Competitiveness
(PID2019-106704RB-100/AEI/10.13039/501100011033)},
organization = {Plan Andaluz de Investigación (Research Group BIO
188)},
organization = {University of Granada Programme
(Empleo Garantía Juvenil)},
organization = {Spanish Ministerio de Educación, Cultura y
Deporte (FPU15/01717)},
publisher = {Frontiers},
keywords = {Peribacillus castrilensis},
keywords = {River otter},
keywords = {New species},
keywords = {Plant-growth promoting species},
keywords = {Phytopathogen},
keywords = {Quorum quenching},
title = {Peribacillus castrilensis sp. nov.: A Plant-Growth-Promoting and Biocontrol Species Isolated From a River Otter in Castril, Granada, Southern Spain},
doi = {10.3389/fpls.2022.896728},
author = {Rodríguez González, Miguel Ángel and Reina Cabello, José Carlos and Sampedro Quesada, María Inmaculada and Llamas Company, Inmaculada and Martínez-Checa Barrero, Fernando José},
}