@misc{10481/76025,
year = {2022},
month = {3},
url = {http://hdl.handle.net/10481/76025},
abstract = {Studying interactions between potential anticancer drugs and cell membrane models is of great interest to explore the capability of novel drugs in the development of anticancer treatments. Lipid membrane models are useful to understand cellular interactions and to discern drug mechanism action. Here, the interactions of curcumin, as a bioactive natural compound with anti-cancer properties, with both healthy and cancerous or tumor cell membrane models, based on Langmuir monolayers, have been studied. The healthy-cell membrane model is composed of cholesterol 67%, and saturated lipid dipalmitoylphosphatidylcholine 33%. The cancerous-cell-membrane-model is composed of a lower proportion of cholesterol, 25%, and unsaturated lipid sphingomyelin 75%. To compare their interaction with curcumin we report the compression isotherms registered for both lipid membrane models and curcumin in different proportions, their compression moduli and the thermodynamic interaction parameters. From this analysis, we evidence a destabilizing interaction between curcumin and the cancerous cell membrane model in comparison with the healthy one. This interaction is further visualized by micro-Brewster Angle and Atomic Force Microscopies. Our experiments show that the drug enhances cohesion in the healthy membrane model whereas it fluidifies the cancerous cell membrane model causing thermodynamic destabilization. These are useful results to improve the selectivity of the drug avoiding adverse side effects of most current anticancer therapies.},
organization = {This work has been supported by project RTI2018-101309-B-C21 funded by MCIN/AEI/10.13039/501100011033/FEDER. María Pedrosa Bustos thanks the FPU19/02045 fellowship funded by MCIN/AEI/10.13039/501100011033 and FSE. This work has been done in the framework of the doctoral of AAG in the Doctoral Programme in Physics and Space Sciences (B09/56/1) of the University of Granada. JMV acknowledges support from project PID2020-116615RA-I00 funded by MCIN/ AEI /10.13039/501100011033. This work was also partially supported by the Biocolloid and Fluid Physics Group (ref. PAI-FQM115) of the University of Granada (Spain). Funding for open access charge: Universidad de Granada / CBUA.},
keywords = {Monolayer},
keywords = {Monocapas},
keywords = {Cell membranes},
keywords = {Membrana celular},
keywords = {Membranas celulares},
keywords = {Curcumin},
keywords = {Curcumina},
keywords = {Langmuir monolayers},
keywords = {Monocapas de Langmuir},
keywords = {Micro BAM},
keywords = {Brewster Angle Microscopy},
keywords = {AFM},
keywords = {Atomic force microscopy},
keywords = {Microscopio de fuerza atómica},
keywords = {DPPC},
keywords = {Esfingomielina},
keywords = {Sphingomyelin},
title = {Interactions between curcumin and cell membrane models by Langmuir monolayers},
doi = {10.1016/j.colsurfb.2022.112636},
author = {Pedrosa Bustos, María and Maldonado Valderrama, Julia and Gálvez Ruiz, María José},
}