@misc{10481/76019,
year = {2022},
month = {5},
url = {http://hdl.handle.net/10481/76019},
abstract = {Bisphenol A (BPA) and its substitutes, bisphenol F (BPF) and S (BPS), have previously shown in vitro obesogenic activity. This study was designed to investigate their combined effect on the adipogenic differentiation of human adipose-derived stem cells (hASCs). Cells were exposed for 14 days to an equimolar mixture of bisphenols (MIX) (range 10 nM-10 mu M). Oil Red staining was used to measure intracellular lipid accumulation, quantitative real-time polymerase chain reaction (qRT-PCR) to study gene expression of adipogenic markers (PPAR gamma, C/EBP alpha, LPL, and FABP4), and Western Blot to determine their corresponding proteins. The MIX promoted intracellular lipid accumulation in a dose-dependent manner with a maximal response at 10 mu M. Co-incubation with pure antiestrogen (ICI 182,780) inhibited lipid accumulation, suggesting that the effect was mediated by the estrogen receptor. The MIX also significantly altered the expression of PPAR gamma, C/EBP alpha, LPL, and FABP4 markers, observing a non-monotonic (U-shaped) dose-response, with maximal gene expression at 10 nM and 10 mu M and lesser expression at 1 mu M. This pattern was not observed when bisphenols were tested individually. Exposure to MIX (1-10 mu M) also increased all encoded proteins except for FABP4, which showed no changes. Evaluation of the combined effect of relevant chemical mixtures is needed rather than single chemical testing.},
organization = {European Commission	733032},
organization = {Biomedical Research Networking CenterCIBER de Epidemiologia y Salud Publica (CIBERESP) of the Institute of Health Carlos III		
European Commission	FIS-PI16/01820	
FIS-PI16/01812	
FIS-PI16/01858},
organization = {Spanish Government	FPU 17/01848},
publisher = {MDPI},
keywords = {Mixtures},
keywords = {Bisphenols},
keywords = {Bisphenol A (BPA)},
keywords = {Bisphenol S (BPS)},
keywords = {Bisphenol F (BPF)},
keywords = {Endocrine disruptors},
keywords = {Dose addition},
title = {The Mixture of Bisphenol-A and Its Substitutes Bisphenol-S and Bisphenol-F Exerts Obesogenic Activity on Human Adipose-Derived Stem Cells},
doi = {10.3390/toxics10060287},
author = {Reina Pérez, Iris and Olivas Martínez, Alicia and Mustieles Miralles, Vicente and Salamanca Fernández, Elena and Molina Molina, José Manuel and Olea Serrano, Nicolás and Fernández Cabrera, Mariana Fátima},
}