@misc{10481/75975,
year = {2022},
month = {6},
url = {http://hdl.handle.net/10481/75975},
abstract = {Bioengineered autologous skin substitutes (BASS) technology is an emerging field for
skin burn therapy. However, further studies on BASS characterization, viability against standard
procedures for wound healing, and protocol optimization are necessary for the improvement of BASS
technology for clinical use. The aim of this study is to evaluate the effect of common antiseptics
for clinical use in BASS, focusing on cell viability, inflammatory cytokine pattern, and epithelium
and skin barrier integrity, in order to establish the most adequate treatment for wound care after
BASS grafting. Human keratinocytes (hKT) and dermal fibroblasts (hDF) were isolated from foreskin
samples and integrated into hyaluronic acid-based BASS. The following antiseptics were applied
every 48 h: ethanol (70%), chlorhexidine digluconate (1%), sodium hypochlorite (0.02%), povidone
iodine (100 mg/mL), and polyhexanide (0.1%), during a follow-up of 16 days. Sodium hypochlorite
was the only treatment that showed a high cell viability percentage throughout the evaluation
time compared to other antiseptic treatments, as well as a similar cytokine secretion pattern as
control BASS. No significant differences were found regarding epidermal barrier function. These
findings point towards sodium hypochlorite being the least aggressive antiseptic treatment for BASS
post-transplantation wound care.},
organization = {Ministry of Health and Families of the Andalusian Regional Government	PIGE-0242-2019	
Instituto de Salud Carlos III	PI17/02083	
BOE22/10/2019},
organization = {Spanish Government},
publisher = {MDPI},
keywords = {Bioengineered autologous skin substitute},
keywords = {Cell viability},
keywords = {Cytokine secretion},
keywords = {Drug development},
keywords = {Epidermal barrier function},
keywords = {In vitro model},
keywords = {Regenerative medicine},
keywords = {Tissue engineering},
title = {Cytotoxicity, Epidermal Barrier Function and Cytokine Evaluation after Antiseptic Treatment in Bioengineered Autologous Skin Substitute},
doi = {10.3390/biomedicines10061453},
author = {García Valdivia, Marta and Quiñones Vico, María Isabel and Ortega Llamas, Laura and Fernández González, Ana and Ubago Rodríguez, Ana Dolores and Sanabria de la Torre, Raquel and Arias Santiago, Salvador Antonio},
}