@misc{10481/75921,
year = {2022},
month = {6},
url = {http://hdl.handle.net/10481/75921},
abstract = {Maslinic acid (MA) is a plant-derived, low water-soluble compound with antitumor activity. We have
formulated MA in the form of solid lipid nanoparticles (SLNs) with three different shell compositions:
Poloxamer 407 (PMA), dicarboxylic acid-Poloxamer 407 (PCMA), and HA-coated PCMA (PCMA-HA).
These SLNs improved the solubility of MA up to 7.5mg/mL, are stable in a wide range of pH, and
increase the bioaccessibility of MA after in vitro gastrointestinal (GI) digestion. Gastrointestinal digested
SLNs afforded MA delivery across in vitro gut barrier models (21 days old Caco-2 and mucus-producing
Caco-2/HT29-MTX co-cultures). The cellular fraction of Caco-2/HT29-MTX co-cultures retained more MA
from GI digested PCMA-HA than the Caco-2 monolayers. The concentration of MA reached in the
basolateral chamber inhibited growth of pancreatic cancer cells, BxPC3. Finally, confocal microscopy
images provided evidence that Nile Red incorporated in MA SLNs was capable of crossing Caco-2
monolayers to be taken up by basolaterally located BxPC3 cells. We have demonstrated that SLNs can
be used as nanocarriers of hydrophobic antitumor compounds and that these SLNs are suitable for
oral consumption and delivery of the bioactive across the gut barrier.},
organization = {Ministerio de Ciencia e Innovacion (MCIN/AEI/FEDER)	RTI2018-101309-B-C21	
RTI2018-101309-B-C22},
organization = {European Molecular Biology Organization (EMBO-short term fellowships)	8475},
organization = {Enterprise Ireland (Career-FIT)},
organization = {European Commission	713654},
organization = {MF2018-0151 Food-BIBS},
organization = {Science Foundation Ireland},
organization = {Department of Agriculture, Food and Marine on behalf of the Government of Ireland	16/RC/3835},
organization = {European Commission
European Commission Joint Research Centre		
European Regional Development Funds of the Junta de Andalucia},
organization = {Spanish Government},
publisher = {Taylor & Francis},
keywords = {Solid lipid nanoparticle},
keywords = {Maslinic acid},
keywords = {Digestion},
keywords = {Bioaccessibility},
keywords = {Intestinal permeability},
title = {Solid lipid nanoparticles to improve bioaccessibility and permeability of orally administered maslinic acid},
doi = {10.1080/10717544.2022.2086937},
author = {Aguilera Garrido, Aixa María and Gálvez Ruiz, María José and Marchal Corrales, Juan Antonio and Galisteo González, Francisco},
}