@misc{10481/75746,
year = {2022},
month = {6},
url = {http://hdl.handle.net/10481/75746},
abstract = {Colorectal cancer (CRC) is one of most common cancers worldwide, with high rates
of mortality. Epidemiological findings demonstrate that coffee consumption reduces the risk of
developing CRC by ~13%. In general, in vivo and in vitro findings demonstrate the antiproliferative,
antioxidant and proapoptotic effects of brewed coffee or major bioavailable coffee compounds.
Thus, it was assessed whether caffeine (CAF) and/or chlorogenic acid (CGA) attenuates the earlystage
of chemically induced mouse colon carcinogenesis. Male Swiss mice were submitted to a
1,2-dimethylhydrazine/deoxycholic acid (DMH/DCA)-induced colon carcinogenesis model. These
animals received CAF (50 mg/kg), CGA (25 mg/kg) or CAF+CGA (50 + 25 mg/kg) intragastrically for
five times/week for ten weeks. CAF+CGA had the most pronounced effects on decreasing epithelial
cell proliferation (Ki-67) and increasing apoptosis (cleaved caspase-3) in colonic crypts. This treatment
also decreased the levels of proinflammatory cytokines IL-6, IL-17 and TNF- , and downregulated
the oncomiR miR-21a-5p in the colon. Accordingly, the analysis of miR-21a-5p targets demonstrated
the genes involved in the negative regulation of proliferation and inflammation, and the positive
regulation of apoptosis. Ultimately, CAF+CGA attenuated preneoplastic aberrant crypt foci (ACF)
development. Our findings suggest that a combination of coffee compounds reduces early-stage
colon carcinogenesis by the modulation of miR-21a-5p expression, highlighting the importance of
coffee intake to prevent CRC.},
organization = {Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)	001},
organization = {Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP)	2017/26217-7	
2016/12015-0	
2016/14420-0},
organization = {Junta de Andalucia	RH-0139-2020},
publisher = {MDPI},
keywords = {Caffeine},
keywords = {Chlorogenic acid},
keywords = {Chemically induced colon carcinogenesis},
keywords = {miRNA expression},
keywords = {Mice},
title = {Caffeine and Chlorogenic Acid Combination Attenuate Early-Stage Chemically Induced Colon Carcinogenesis in Mice: Involvement of oncomiR miR-21a-5p},
doi = {10.3390/ijms23116292},
author = {Rocha Bartolomeu, Ariane and Griñán Lisón, Carmen and Marchal Corrales, Juan Antonio and García Chaves, María Ángel},
}