@misc{10481/75672,
year = {2022},
month = {5},
url = {http://hdl.handle.net/10481/75672},
abstract = {There is a global need to discover effective anti-cancerous compounds from natural sources.
Cultivated wheat cells can be a valuable source of non-toxic or low toxic plant-derived polysaccharides.
In this study, we evaluated the anti-cancer ability of seven fractions of wheat cell culture
polysaccharides (WCCPSs) in the HCT-116 colon cancer cell line. Almost all (6/7) fractions had
an inhibitory effect on the proliferation of colon cancer cells, and two fractions (A-b and A-f) had
considerable therapeutic indexes. The WCCPS fractions induced cell cycle arrest in the G1 phase
and induced different rates of apoptosis ( 48%). Transmission and scanning electron microscopy
revealed that WCCPS fractions caused apoptotic changes in the nucleus and cytoplasm, including
damage to mitochondria and external morphological signs of apoptosis. In addition, the WCCPSs
induced an increase in the levels of Bax, cytochrome c, and caspases 8 and 3, indicating that cell
death progressed through intrinsic and extrinsic pathways of apoptosis. Furthermore, some fractions
caused a significant decrease of c-Myc, b-catenin, NFkB2, and HCAM (CD 44) levels, indicating
enhanced cell differentiation. Thus, for the first time, our results provide a proof of concept of the
anti-cancer capacity of WCCPS fractions in colorectal cancer.},
organization = {FEDER Operational Program 2020/Junta de AndaluciaConsejeria de Economia y Conocimiento/Project	B-CTS-562-UGR20},
organization = {Chair "Doctors GaleraRequena in cancer stem cell research"	CMC-CTS963},
organization = {Erasmus+ Mobility Program},
organization = {Republic of Kazakhstan},
organization = {Scottish Government's Rural and Environment Science and Analytical Services (RESAS) division},
publisher = {MDPI},
keywords = {Wheat cell culture},
keywords = {Plant polysaccharides},
keywords = {Colon cancer cells},
keywords = {Inhibition of proliferation},
keywords = {Electron microscopy},
keywords = {Apoptosis},
keywords = {Cytochrome c},
keywords = {Differentiation},
title = {Anti-Cancerous Potential of Polysaccharides Derived from Wheat Cell Culture},
doi = {10.3390/pharmaceutics14051100},
author = {Murtazina, Alima and Ruiz Alcalá, Gloria and Jiménez Martínez, Yaiza and Marchal Corrales, Juan Antonio and Boulaiz Tassi, Houria},
}