@misc{10481/74660,
year = {2022},
month = {3},
url = {http://hdl.handle.net/10481/74660},
abstract = {The paradigm of mast cells in type 2 diabetes is changing. Although they were first
considered deleterious inflammatory cells, now they seem to be important players driving
adipose tissue homeostasis. Here we have employed a flow cytometry-based approach
for measuring the surface expression of 4 proteins (CD45, CD117, CD203c, and FcϵRI) on
mast cells of omental (o-WAT) and subcutaneous white adipose tissue (s-WAT) in a cohort
of 96 patients with morbid obesity. The cohort was split into three groups: non-T2D, pre-
T2D, and T2D. Noteworthy, patients with T2D have a mild condition (HbA1c <7%). In o-
WAT, mast cells of patients with T2D have a decrease in the surface expression of CD45
(p=0.0013), CD117 (p=0.0066), CD203c (p=0.0025), and FcϵRI (p=0.043). Besides, in s-
WAT, the decrease was seen only in CD117 (p=0.046). These results indicate that T2D
affects more to mast cells in o-WAT than in s-WAT. The decrease in these four proteins
has serious effects on mast cell function. CD117 is critical for mast cell survival, while
CD45 and FcϵRI are important for mast cell activation. Additionally, CD203c is only
present on the cell surface after granule release. Taking together these observations, we
suggest that mast cells in o-WAT of patients with T2D have a decreased survival,
activation capacity, and secretory function.},
organization = {Instituto de Salud Carlos III},
organization = {European Commission	PI15/01361	
PI18/01947},
organization = {Junta de Andalucia	FPU18/04432	
PIN-0235-2019},
organization = {Spanish Ministry of Health. ISCIII funds},
organization = {Junta de Andalucia		
B16.56.1},
publisher = {Frontiers},
keywords = {Mast cell},
keywords = {Adipose tissue},
keywords = {Obesity},
keywords = {Type 2 diabetes},
keywords = {Flow cytometry},
keywords = {Bariatric surgery},
title = {In Obese Patients With Type 2 Diabetes, Mast Cells in Omental Adipose Tissue Decrease the Surface Expression of CD45, CD117, CD203c, and FcϵRI},
doi = {10.3389/fendo.2022.818388},
author = {López Pérez, David and Redruello Romero, Anaïs and García Rubio, Jesús and Sánchez Tamayo, Francisco Javier and Salmerón Escobar, Francisco Javier and Gálvez Peralta, Julio Juan and León, Josefa and Carazo Gallego, Ángel},
}