@misc{10481/74404,
year = {2022},
month = {3},
url = {http://hdl.handle.net/10481/74404},
abstract = {Lung cancer is the most common cancer in the world and several miRNAs are associated
with it. MiRNA sponges are presented as tools to inhibit miRNAs. We designed a system to capture
miRNAs based on circular RNAs (circRNA). To demonstrate its usefulness, we chose miR-21, which is
upregulated and implicated in lung cancer. We constructed a miR-21 sponge and inserted it into
a vector that facilitates circular RNA production (Circ-21) to study its effect on growth, colony
formation, and migration in lung cancer cell lines and multicellular tumor spheroids (MTS). Circ-21
induced a significant and time-dependent decrease in the growth of A549 and LL2 cells, but not
in L132 cells. Furthermore, A549 and LL2 cells transfected with Circ-21 showed a lower number
of colonies and migration than L132. Similar findings were seen in A549 and LL2 Circ-21 MTS,
which showed a significant decrease in volume growth, but not in L132 Circ-21 MTS. Based on this,
the miR-21 circular sponge may suppress the processes of tumorigenesis and progression. Therefore,
our system based on circular sponges seems to be effective, as a tool for the capture of other miRNAs.},
organization = {Instituto de Salud Carlos III
European Commission	PI19/01478},
organization = {CTS-107},
publisher = {MDPI},
keywords = {miRNA},
keywords = {Circular sponges},
keywords = {miRNA-21},
keywords = {Lung cancer},
title = {Synthetic Circular miR-21 Sponge as Tool for Lung Cancer Treatment},
doi = {10.3390/ijms23062963},
author = {Rama Ballesteros, Ana Rosa and Quiñonero Muñoz, Francisco José and Mesas Hernández, Cristina and Melguizo Alonso, Consolación and Prados Salazar, José Carlos},
}