@misc{10481/74094,
year = {2022},
month = {1},
url = {http://hdl.handle.net/10481/74094},
abstract = {Introduction: Paclitaxel (PTX) is a cornerstone in the
treatment of breast cancer, the most common type
of cancer in women. However, this drug has serious
limitations, including lack of tissue-specificity,
poor water solubility, and the development of drug
resistance. The transport of PTX in a polymeric
nanoformulation could overcome these limitations.
Methods: In this study, PLGA-PTX nanoparticles
(NPs) were assayed in breast cancer cell lines, breast
cancer stem cells (CSCs) and multicellular tumor
spheroids (MTSs) analyzing cell cycle, cell uptake (Nile Red-NR-) and α-tubulin expression. In
addition, PLGA-PTX NPs were tested in vivo using C57BL/6 mice, including a biodistribution
assay.
Results: PTX-PLGA NPs induced a significant decrease in the PTX IC50 of cancer cell lines (1.31
and 3.03-fold reduction in MDA-MB-231 and E0771 cells, respectively) and CSCs. In addition,
MTSs treated with PTX-PLGA exhibited a more disorganized surface and significantly higher
cell death rates compared to free PTX (27.9% and 16.3% less in MTSs from MCF-7 and E0771,
respectively). PTX-PLGA nanoformulation preserved PTX’s mechanism of action and increased
its cell internalization. Interestingly, PTX-PLGA NPs not only reduced the tumor volume of treated
mice but also increased the antineoplastic drug accumulation in their lungs, liver, and spleen. In
addition, mice treated with PTX-loaded NPs showed blood parameters similar to the control mice,
in contrast with free PTX.
Conclusion: These results suggest that our PTX-PLGA NPs could be a suitable strategy for breast
cancer therapy, improving antitumor drug efficiency and reducing systemic toxicity without
altering its mechanism of action.},
organization = {V Plan Propio (University of Seville)},
organization = {Junta de Andalucia	PI-0102-2017	
P18-HO-3882},
organization = {Instituto de Salud Carlos III},
organization = {European Commission	PI19/01478},
publisher = {Tabriz University of Medical Sciences and Health Services},
keywords = {Paclitaxel},
keywords = {PLGA},
keywords = {Breast cancer},
keywords = {Cancer stem cells},
keywords = {Mice xenografits},
title = {Evaluation of poly (lactic-co-glycolic acid) nanoparticles to improve the therapeutic efficacy of paclitaxel in breast cancer},
doi = {10.34172/bi.2022.23433},
author = {Cabeza Montilla, Laura and Ortiz Quesada, Raúl and Jiménez López, Julia and Perazzoli, Gloria and Baeyens Cabrera, José Manuel and Melguizo Alonso, Consolación and Prados Salazar, José Carlos},
}