@misc{10481/73899,
year = {2022},
month = {3},
url = {http://hdl.handle.net/10481/73899},
abstract = {Introduction: A large body of evidence suggests that propolis exerts antioxidant, anti-inflammatory, and antimicrobial
activities, mostly ascribed to its polyphenol content. Growing evidence suggests that propolis could
modulate gut microbiota exerting a positive impact on several pathological conditions. The aim of this study was
to determine the in vitro impact of a poplar-type propolis extract with a standardized polyphenol content, on the
composition and functionality of gut microbiota obtained from fecal material of five different donors (healthy
adults, and healthy, obese, celiac, and food allergic children).
Methods: The standardized polyphenol mixture was submitted to a simulated in vitro digestion-fermentation
process, designed to mimic natural digestion in the human oral, gastric, and intestinal chambers. The antioxidant
profile of propolis before and after the digestion-fermentation process was determined. 16 S rRNA amplicon
next-generation sequencing (NGS) was used to test the effects on the gut microbiota of propolis extract. The
profile of the short-chain fatty acids (SCFA) produced by the microbiota was also investigated through a chromatographic
method coupled with UV detection.
Results: In vitro digestion and fermentation induced a decrease in the antioxidant profile of propolis (i.e., decrease
of total polyphenol content, antiradical and reducing activities). Propolis fermentation exhibited a modulatory
effect on gut microbiota composition and functionality of healthy and diseased subjects increasing the concentration
of SCFA.
Conclusions: Overall, these data suggest that propolis might contribute to gut health and could be a candidate for
further studies in view of its use as a prebiotic ingredient.},
organization = {Plan Propio de Investigación y
Transferencia of the University of Granada, Spain under the program
“Intensificación de la Investigación, modalidad B},
publisher = {Elsevier},
keywords = {Poplar-type propolis},
keywords = {Multi dynamic extraction},
keywords = {In vitro digestion},
keywords = {In vitro fermentation},
keywords = {Antioxidant capacity},
keywords = {Gut microbiota},
keywords = {Short-chain fatty acids},
title = {Evaluating the effects of a standardized polyphenol mixture extracted from poplar-type propolis on healthy and diseased human gut microbiota},
doi = {10.1016/j.biopha.2022.112759},
author = {Garzarella, E. U. and Navajas Porras, Beatriz and Pérez Burillo, Sergio and Hinojosa Nogueira, Daniel José and Pastoriza de la Cueva, Silvia and Rufián Henares, José Ángel and Daglia, Maria},
}