@misc{10481/73185,
year = {2021},
month = {12},
url = {http://hdl.handle.net/10481/73185},
abstract = {Erythrodiol (EO) is a pentacyclic triterpenic alcohol found in olive tree leaves and olive
oil, and it has important effects on the health properties and quality of olive oil. In this study, we
characterized the cytotoxic effects of EO on human hepatocarcinoma (HepG2) cells by studying
changes in cell viability, reactive oxygen species (ROS) production, antioxidant defense systems, and
the proteome. The results reveal that EO markedly decreased HepG2 cell viability without changing
ROS levels. The concentrations of glutathione and NADPH were significantly reduced, with selective
changes in the activity of several antioxidant enzymes: glutathione peroxidase, glutathione reductase,
glucose 6-phosphate dehydrogenase, and 6-phosphogluconate dehydrogenase. Proteomic data reveal
that EO led to the complete elimination or decreased abundance of 41 and 3 proteins, respectively,
and the abundance of 29 proteins increased. The results of functional enrichment analysis show that
important metabolic processes and the nuclear transport of mature mRNA were impaired, whereas
AMP biosynthesis and cell cycle G2/M phase transition were induced. The transcription factors and
miRNAs involved in this response were also identified. These potent antiproliferative effects make
EO a good candidate for the further analysis of its hepatic antitumor effects in in vivo studies.},
organization = {University of Jaén (Plan Propio de Investigación, grant
number UJA2014/07/13)},
organization = {Junta de Andalucía (Plan Andaluz de Investigación, Junta de
Andalucía, Spain), grant BIO-341},
publisher = {MDPI},
keywords = {Erythrodiol},
keywords = {HepG2},
keywords = {Cytotoxicity},
keywords = {ROS},
keywords = {Antioxidant enzymes},
keywords = {GSH},
keywords = {NADPH},
keywords = {Proteome},
title = {Effects of Erythrodiol on the Antioxidant Response and Proteome of HepG2 Cells},
doi = {10.3390/antiox11010073},
author = {Peñas Fuentes, Juan Luis and Rufino Palomares, Eva and Pérez Jiménez, Amalia and Reyes Zurita, Fernando Jesús and Lupiáñez Cara, José Antonio},
}