@misc{10481/72957,
year = {2021},
month = {12},
url = {http://hdl.handle.net/10481/72957},
abstract = {Regulatory interactions between enhancers and core
promoters are fundamental for the temporal and spatial
specificity of gene expression in development.
The central role of core promoters is to initiate productive
transcription in response to enhancer’s activation
cues. However, it has not been systematically
assessed how individual core promoter elements affect
the induction of transcriptional bursting by enhancers.
Here, we provide evidence that each core
promoter element differentially modulates functional
parameters of transcriptional bursting in developing
Drosophila embryos. Quantitative live imaging
analysis revealed that the timing and the continuity
of burst induction are common regulatory steps
on which core promoter elements impact. We further
show that the upstream TATA also affects the
burst amplitude. On the other hand, Inr, MTE and
DPE mainly contribute to the regulation of the burst
frequency. Genome editing analysis of the pair-rule
gene fushi tarazu revealed that the endogenous TATA
and DPE are both essential for its correct expression
and function during the establishment of body
segments in early embryos. We suggest that core
promoter elements serve as a key regulatory module
in converting enhancer activity into transcription
dynamics during animal development.},
organization = {Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)},
organization = {Japan Society for the Promotion of Science	18H06040	
20K15710	
21K20627},
organization = {Ministry of Education, Culture, Sports, Science and Technology, Japan (MEXT)},
organization = {Tomizawa Jun-ichi & Keiko Fund of Molecular Biology Society of Japan for Young Scientist},
organization = {Mochida Memorial Foundation for Medical and Pharmaceutical Research},
organization = {Nakajima Foundation},
organization = {Inamori Foundation},
organization = {Takeda Science Foundation (TSF)},
organization = {Sumitomo Foundation},
organization = {Senri Life Science Foundation},
organization = {Mitsubishi Foundation},
organization = {University of Tokyo},
organization = {JST, ACT-X	JPMJAX211J},
organization = {Spanish Government	FPI2015/074837},
organization = {University of Granada		
21H05742	
20H05357	
19H03154	
19K22378},
publisher = {Oxford University Press},
title = {Dynamic modulation of enhancer responsiveness by core promoter elements in living Drosophila embryos},
doi = {10.1093/nar/gkab1177},
author = {Yokoshi, Moe and Cambón Gandarias, Manuel},
}